Recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM)
白細胞激活黏附因子(ALCAM)重組蛋白
[ PROPERTIES ]
Residues: Gly225~Ala478,with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Membrane; Single-passtype I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 29.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8℃ for one month.
Aliquot and store at -80℃ for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37℃ for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.
MGHHHHHHSGSEF-GQPEGI RSSNTYTLPN VRRNATGNYK CSLIDKKSLI ASTAITVHYLDLSLNPXGEL TKQIGDSLPV SCTISAIRNA TVVWMKDNIK LRSSPSFSSL QYQDAGNYVCETALQEVEGL KKRESLTLIV EVKPQIKMTK KTDPSGLSKT IICHVEGFPK PAIQWTITGSGSVINQTEES PYINGRYYSK IIISPEENVT LTCAAENQLE RTVNSLNVSA ISIPEHDEADEISDENREKV NDQAKLIVGI VVGLLLAA
[ IDENTIFICATION ]
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關鍵字： (ALCAM);
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