重組羧肽酶B（EC 3.4.17.2）是一種金屬依賴性外切蛋白酶，通過基因工程技術(shù)在大腸桿菌或酵母菌中表達并純化，特異性水解多肽鏈C -末端的堿性氨基酸（賴氨酸Lys、精氨酸Arg、組氨酸His），是重組胰島素及類似物生產(chǎn)、蛋白質(zhì)測序與修飾、生物制藥工藝優(yōu)化的核心工具酶

Recombinant carboxypeptidase B (EC 3.4.17.2) is a metal?dependent exopeptidase that is expressed and purified from E. coli or yeast using genetic engineering techniques. It specifically hydrolyzes basic amino acids (lysine, arginine, histidine) at the C?terminus of polypeptide chains. It serves as a core tool enzyme in the production of recombinant insulin and its analogs, protein sequencing and modification, and biopharmaceutical process optimization.