Recombinant Aquaporin 4 (AQP4)
水通道蛋白4(AQP4)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Asp179~Asp314
Tags: Two N-terminal Tags, His-tag and GST-tag
Tissue Specificity: Brain, Kidney, Lung.
Subcellular Location: Membrane; Multi-pass membrane protein.
Purity: > 98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 45.0kDa
Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducingconditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
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關鍵字： (AQP4);
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