產品描述:
Fibroblast Growth Factor-basic (bFGF) is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
表達系統(tǒng) :
Escherichia coli
溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
產品組成:
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
存儲條件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
分子量:
Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
內毒素:
Less than 1 EU/μg of bFGF as determined by LAL method.
產品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.
生物學活性:
The ED50 determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1.8 ng/mL, corresponding to a specific activity of > 5.6 × 105 IU/mg.
產品形態(tài):
Sterile filtered white lyophilized (freeze-dried) powder.
氨基酸序列:
MPALPEDGGG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
產品純度:
> 98% by SDS-PAGE and HPLC analyses.
關鍵字： 大鼠堿性成纖維細胞生長因子 (BFGF) (RAT)
賽業(yè)（蘇州）生物科技有限公司是賽業(yè)生物集團2011年在蘇州太倉市投資興建的模式動物生產基地，定位于模式動物的研發(fā)與生產。賽業(yè)生物2006年成立于美國，在中國設立有賽業(yè)蘇州公司和賽業(yè)廣州公司，在北京、上海、武漢、重慶等地均有辦事處。目前員工人數(shù)達400余名，總規(guī)模超22000平方米，業(yè)務經營范圍涵蓋模式動物、分子生物學、細胞生物學、生物信息學等生命科學領域的多個支學科。