Protein Name

Human cysteine protease

Protein Symbol

ATG4A

Original Source

Expression System

GenBank Accession #

NM_052936.1

Uniprot Accession #

Q8WYN0

Description

Cysteine protease ATG4A belongs to a member of the autophagin protein family. This protein is also designated as a member of the C-54 family of cysteine proteases. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. Cysteine protease ATG4A cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.

Application

WB, ELISA, IP, antibody production, protein array

Fusion tag

N-His

Peptide Length

413aa(including fusion tag)

Molecular Weight

47.3kDa(including fusion tag)

pI

5.1

Activity

NA

Storage

Storage buffer: 20mM Tris.Cl, 100mM NaCl, 0.4M Urea, 50% Glycerol, pH8.0. Store at -80°C and avoid repeated freeze-thaw cycles.