Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

18133

Q64299

E. coli

333aa

36.4kDa

－

About this Antibody

Name

Recombinant Murine NOV (Recombinant Murine Nephroblastomaoverexpressed Gene Protein Homolog; rMuNOV)；Recombinant Mouse NOV ; 重組小鼠成腎腎母細胞瘤過度表達基因同源蛋白

Synonyms

CCN3; IGFBP-9; CCN family member 3; CCN3IGF-binding protein 9; IBP-9; IGFBP-9; IGFBP9novH; Insulin-like growth factor-binding protein 9; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOVH; NovH; protein NOV homolog

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0μg/ml, corresponding to a specific activity of >1000IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH8.6, 150mM NaCl.

Endotoxin

Less than 0.1EU/μg of rMuNOV as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

NOV, also named CCN3, IGFBP-9 and NovH, is belonging to the CCN (CTGF/CYR61/NOV) family and it is encoded by the NOV gene. It is expressed in certain tumors, including Wilm's tumor and most nephroblastomas. NOV interacts with several proteins and is involved in both internal and external cell signaling. NOV plays an important role in reducing tumorgenicity and proliferation of certain cancer cell lines. It also been reported to exert proangiogenic activities. Recombinant murine NOV is an 36.4kDa protein containing 333 amino acid residues and it shares 92% and 82%a.a. identity with rat and human NOV.

Amino Acid Sequence

QVSASLRCPS RCPPKCPSIS PTCAPGVRSV LDGCSCCPVC ARQRGESCSE MRPCDQSSGL YCDRSADPNN QTGICMVPEG DNCVFDGVIY RNGEKFEPNC QYFCTCRDGQ IGCLPRCQLD VLLPGPDCPA PRKVAVPGEC CEKWTCGSDE QGTQGTLGGL ALPAYRPEAT VGVEVSDSSI NCIEQTTEWS ACSKSCGMGV STRVTNRNRQ CEMVKQTRLC IVRPCEQEPE EVTDKKGKKC LRTKKSLKAI HLQFENCTSL YTYKPRFCGV CSDGRCCTPH NTKTIQVEFQ CLPGEIIKKP VMVIGTCTCY SNCPQNNEAF LQDLELKTSR GEI

產(chǎn)品編號

產(chǎn)品名稱

包裝

P6110-5μg

Recombinant Murine NOV

5μg

P6110-20μg

Recombinant Murine NOV

20μg

P6110-100μg

Recombinant Murine NOV

100μg

P6110-1mg

Recombinant Murine NOV

1mg

-

說明書

1份