| 價格 | 詢價 |
| 包裝 | 10μg |
| 最小起訂量 | 10μg |
| 發(fā)貨地 | 上海 |
| 更新日期 | 2026-06-09 |
| 中文名稱:Recombinant Human TNF-α/TNFSF2, His-tag | 英文名稱:Recombinant Human TNF-α/TNFSF2, His-tag |
| 有效期: 一年 | 產(chǎn)品類別: 重組蛋白 細(xì)胞因子 |
| 產(chǎn)品編號 | 產(chǎn)品名稱 | 產(chǎn)品包裝 | 產(chǎn)品價格 |
| P5322-10μg | Recombinant Human TNF-α/TNFSF2, His-tag | 10μg | 詢價 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 7124 | P01375 | E. coli | 164aa | 18.3kDa | His |
| About this protein | |
| Name | Recombinant Human TNF-α/TNFSF2, His-tag (Recombinant Human Tumor Necrosis Factor-α/TNFSF2, His-tag; rHuTNF-α/TNFSF2, His-tag);重組人腫瘤壞死因子α/TNFSF2,His標(biāo)簽 |
| Synonyms | Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0. |
| Endotoxin | Less than 1EU/μg of rHuTNF-α/TNFSF2, His-tag as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types. |
| Amino Acid Sequence | MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL |
包裝清單:
| 產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝 |
| P5322-10μg | Recombinant Human TNF-α/TNFSF2, His-tag | 10μg |
| P5322-50μg | Recombinant Human TNF-α/TNFSF2, His-tag | 50μg |
| P5322-100μg | Recombinant Human TNF-α/TNFSF2, His-tag | 100μg |
| P5322-1mg | Recombinant Human TNF-α/TNFSF2, His-tag | 1mg |
| - | 說明書 | 1份 |
保存條件:
???????-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會引起部分失活,所以首次配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液),須分裝后-20℃或更低溫度凍存,以避免反復(fù)凍融。
注意事項(xiàng):
???????由于有些塑料管壁對某些蛋白有較強(qiáng)的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn),使細(xì)胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
???????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
???????為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。
| 成立日期 | 2007-07-19 (19年) | 注冊資本 | 1000.000000萬人民幣 |
| 員工人數(shù) | 500人以上 | 年?duì)I業(yè)額 | ¥ 500萬-1000萬 |
| 主營行業(yè) | 生化試劑,核苷,核苷酸,寡核苷酸,抗體,蛋白組學(xué),生物活性小分子 | 經(jīng)營模式 | 試劑 |
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