| 價(jià)格 | 詢(xún)價(jià) |
| 包裝 | 100μg |
| 最小起訂量 | 100μg |
| 發(fā)貨地 | 上海 |
| 更新日期 | 2026-07-12 |
| 中文名稱(chēng):Recombinant Human LIF | 英文名稱(chēng):Recombinant Human LIF |
| 有效期: 一年 | 產(chǎn)品類(lèi)別: 重組蛋白 細(xì)胞因子 |
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 產(chǎn)品包裝 | 產(chǎn)品價(jià)格 |
| P5354-100μg | Recombinant Human LIF | 100μg | 詢(xún)價(jià) |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 3976 | P15018 | E. coli | 180aa | 19.7kDa | - |
| About this protein | |
| Name | Recombinant Human LIF (Recombinant Human Leukemia Inhibitory Factor; rHuLIF);重組人白血病抑制因子 |
| Synonyms | CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); Melanoma-derived LPL inhibitor; MLPLI |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4. |
| Endotoxin | Less than 1EU/μg of rHuLIF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.2mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature human LIF (180a.a.) shares 78%, 82%, 91%, 88% and 87%a.a. sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. |
| Amino Acid Sequence | SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF |
包裝清單:
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 包裝 |
| P5354-5μg | Recombinant Human LIF | 5μg |
| P5354-25μg | Recombinant Human LIF | 25μg |
| P5354-100μg | Recombinant Human LIF | 100μg |
| P5354-1mg | Recombinant Human LIF | 1mg |
| - | 說(shuō)明書(shū) | 1份 |
保存條件:
???????-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活,所以首次配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液),須分裝后-20℃或更低溫度凍存,以避免反復(fù)凍融。
注意事項(xiàng):
???????由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn),使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
???????本產(chǎn)品僅限于專(zhuān)業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
???????為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
| 成立日期 | 2007-07-19 (20年) | 注冊(cè)資本 | 1000.000000萬(wàn)人民幣 |
| 員工人數(shù) | 500人以上 | 年?duì)I業(yè)額 | ¥ 500萬(wàn)-1000萬(wàn) |
| 主營(yíng)行業(yè) | 生化試劑,核苷,核苷酸,寡核苷酸,抗體,蛋白組學(xué),生物活性小分子 | 經(jīng)營(yíng)模式 | 試劑 |
| 產(chǎn)品名稱(chēng) | 價(jià)格 | 公司名稱(chēng) | 報(bào)價(jià)日期 | |
|---|---|---|---|---|
| ¥646 |
VIP7年
|
上海康朗生物科技有限公司
|
2026-07-27 | |
| ¥3300 |
VIP1年
|
上海撫生實(shí)業(yè)有限公司
|
2026-07-27 | |
| 詢(xún)價(jià) |
VIP7年
|
上?;菡\(chéng)生物科技有限公司
|
2026-07-27 |