| 產品編號 |
產品名稱 |
產品包裝 |
產品價格 |
| AF0222 |
MAPK14 Mouse Monoclonal Antibody |
50μl |
詢價 |
| 來源 |
用途 |
交叉反應性 |
分子量 |
| Mouse |
WB, ICC, IHC, IF |
H, M |
40KDa |
??????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
??????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig;
Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
??????配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
??????建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
| WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
| 1:1000 |
- |
1:20-1:40 |
1:20-1:40 |
1:20-1:40 |
- |
- |
- |
抗體詳細信息如下:
| About this Antibody |
| Name |
MAPK14 Mouse Monoclonal Antibody |
| Category |
Monoclonal antibody(mAb); Primary antibody |
| Isotype |
IgG2a |
| Purification |
ProA affinity purified |
| About the Immunogen |
| Immunogen |
This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of MAPK14. |
| Gene ID |
1432 |
| SwissProt |
Q16539 |
| Synonyms |
MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; EXIP; Mxi2; PRKM14; PRKM15; RK; p38; p38ALPHA |
| Category |
MAP Kinase Signaling |
| Background |
Mitogen-activated protein kinase 14, also called p38-α, is an enzyme that in humans is encoded by the MAPK14 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. |
包裝清單：
| 產品編號 |
產品名稱 |
包裝 |
| AF0222 |
MAPK14 Mouse Monoclonal Antibody |
50μl |
| AZ050 |
Western一抗稀釋液 |
50ml |
| — |
說明書 |
1份 |
保存條件：
??????MAPK14 Mouse Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
??????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
??????回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
??????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
??????本產品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
??????為了您的安全和健康，請穿實驗服并戴一次性手套操作。