Recombinant Mouse T cell Immunoreceptor with Ig and ITIM domains is produced by our Mammalian expression system and the target gene encoding Gly26-Phe135 is expressed with a 6His tag at the C-terminus.
Background
T cell immunoreceptor with Ig and ITIM domains (TIGIT), also called WUCAM, VSIG9 and Vstm3, is a member of the CD28 family within the Ig superfamily of proteins. TIGIT contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM), and is expressed on regulatory, memory, activated T cells and NK cells. TIGIT binds to CD155(PVR) that appear on dendritic cells (DC), macrophages and endothelium with high affinity, and CD112(PVRL2) with lower affinity, but not CD113 (PVRL3). TIGIT-Fc fusion protein could interact with PVR on DC and enhance the secretion of IL-10, but inhibit the macrophage activation. Mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges, while knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses.
Known as
T-cell immunoreceptor with Ig and ITIM domains;TIGIT;V-set and transmembrane domain-containing protein 3; VSTM3
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
關鍵字： Recombinant Mouse TIGIT/VSTM3;細胞生物學
北京索萊寶科技有限公司是一家集研發(fā)、生產、銷售、服務于一體的高科技生物企業(yè)。技術推廣;技術轉讓；技術咨詢；技術服務；生物試劑技術開發(fā)；銷售生物試劑盒、實驗室專用化學試劑、實驗室專用設備、辦公用品、化工產品（不含危險化學品）、計算機軟件及輔助設備；貨物進出口、技術進出口、代理進出口；委托加工生產生物制品、生化制品、化工產品（不含危險化學品）；生產生物試劑盒；批發(fā)第一類醫(yī)療器械、第二類醫(yī)療器械；批發(fā)第三類醫(yī)療器械。