l?Analyze small aliquots from RNA preparations before Northern blotting, start-site mapping or cDNA preparation
l?Visualize the migration behavior of 5S rRNA species after high-resolution denaturing gradient electrophoresis (DGGE)
l?Stain DNA in single-strand conformation polymorphism (SSCP)
l?Stain DNA before amplification by PCR
Materials Contents
SYBR Green II stain is provided as a 10,000X concentrate in DMSO in the following sizes:
l?50 μL (SY1040-50)
l?500 μL (SY1040-500)
Storage
Upon receipt, store the dye frozen at -20°C, protected from light in a desiccator. When stored properly, the SYBR Green II stain stock solution in DMSO is stable for six months to one year.
注：SYBR Green II主要用于RNA和單鏈DNA電泳。是EB的替代品，無(wú)致癌性，超敏感性。用作常規(guī)電泳時(shí)，須將商品化的核酸MARKER作一定倍數(shù)的稀釋（1/5-1/10），這樣才能得到比較理想的結(jié)果。
關(guān)鍵字： SYBR Green II RNA;分子生物學(xué)
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