Name

Recombinant Human ApoA-I (Recombinant Human Apolipoprotein A-I; rHuApoA-I)；重組人載脂蛋白A-I

Synonyms

Alp-1; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; APOA1; Apo-AI; apo-AI; ApoA-I; apoA-I; Apolipoprotein A1; apolipoprotein A-I; MGC117399

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Data Not Available.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 0.1EU/μg of rHuApoA-I as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Apolipoprotein A-I encoded by the APOA1 gene is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Apolipoprotein A-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Apolipoprotein A-I is also isolated as a prostacyclin (PGI2) stabilizing factor and thus may have an anticlotting effect. Apolipoprotein A-I has been shown to interact with ABCA1, GPLD1 and PLTP. Apolipoprotein A-I binds to lipopolysaccharide or endotoxin and has a major role in the anti-endotoxin function of HDL. Mature human ApoA-1 shares 65% and 62%a.a. sequence identity with murine and rat ApoA-1, respectively. It has an approximate molecular weight of 28.1kDa.

Amino Acid Sequence

DEPPQSPWDR VKDLATVYVD VLKDSGRDYV SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SALEEYTKKL NTQ

產(chǎn)品編號

產(chǎn)品名稱

包裝

KL7280-20μg

Recombinant Human ApoA-I

20μg

KL7280-100μg

Recombinant Human ApoA-I

100μg

KL7280-1mg

Recombinant Human ApoA-I

1mg

-

說明書

1份