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Acute Toxicologic Evaluation at p-Anisidine

Dec 1,2023

Eye Irritation

p-Anisidine produced conjunctival injection, chemosis, and colorless diseharge. Miosis was observed from 15 min-2 hrs. Ocular effects disappeared in 1 eye at 72 hrs., but worsened in the other 3 eyes after 48 hrs. with mucoid discharge and iridal congestion. Two of 3 eyes appeared nOrmal at 6 days. One remained irritated at the end of the 14 days. The compound had an average Draize score fOf Day 1= 17, Day 7= 7 an~ Day 14= 7.

Skin Irritation

Two of the 3 abraded sites had slight erythema and 1 site also had a slight amount of edema at 24 hrs. No effects were present at 48 hrs. The remaining abraded and intact sites showed no irritation during the 14 days. The p-Anisidine's primary irritation score= 0.1.

Oral Toxicity

Within 10 min. at all dose levels, ataxia, bradypnea, decreased activity, loss of righting reflex, and exophthalmos occurred. Deaths occured within 30 min- 24 hrs. with 4 late deaths in 2-4 days. LD50 was determined to be 1410 mg/kg.

p-Anisidine reacts with secondary oxidation products such as aldehydes and ketones in fats and oils. P-Anisidine is the most toxic of the three isomers of anisidine and causes blood damage upon oral ingestion, inhalation or skin contact. If heated strongly, it may release very toxic fumes of nitrogen oxides.

References:

[1] C. O’NEAL, W. B, E V Sargent. Acute Toxicologic Evaluation of p-Anisidine[J]. International Journal of Toxicology, 1992, 1 1: 184-185. DOI:10.1177/109158189200100330.

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