How is D(+)-glucose commonly analyzed in quality control?
Apr 1，2026
Typical analytical methods include high-performance liquid chromatography (HPLC) with refractive index detection for purity and content assay, enzymatic methods based on hexokinase or glucose oxidase for high specificity in biological samples, and classic titration methods using Fehling’s reagent or Benedict’s reagent for reducing-sugar determination. Polarimetry is also frequently used to identify and quantify D?glucose via its characteristic optical rotation.
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