一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of virological methods >>article
Journal of virological methods

Journal of virological methods

IF: 2.2
Download PDF

Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein

Published:1 September 2022 DOI: 10.1016/j.jviromet.2022.114564 PMID: 35671888
Yuting Hong , Huilin Guo , Min Wei , Yali Zhang , Mujin Fang , Tong Cheng , Zhiyong Li , Shengxiang Ge , Xiangyang Yao , Quan Yuan , Ningshao Xia

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 infections has led to excess deaths worldwide. Neutralizing antibodies (nAbs) against viral spike protein acquired from natural infections or vaccinations contribute to protection against new- and re-infections. Besides neutralization, antibody-mediated cellular cytotoxicity (ADCC) and phagocytosis (ADCP) are also important for viral clearance. However, due to the lack of convenient methods, the ADCC and ADCP responses elicited by viral infections or vaccinations remain to be explored. Here, we developed cell-based assays using target cells stably expressing SARS-CoV-2 spikes and Jurkat-NFAT-CD16a/CD32a effector cells for ADCC/ADCP measurements of monoclonal antibodies and human convalescent COVID-19 plasmas (HCPs). In control samples (n?=?190), the specificity was 99.5% (95%CI: 98.4–100%) and 97.4% (95%CI: 95.1–99.6%) for the ADCC and ADCP assays, respectively. Among 87 COVID-19 HCPs, 83 (sensitivity: 95.4%, 95%CI: 91.0–99.8%) and 81 (sensitivity: 93.1%, 95%CI: 87.8–98.4%) showed detectable ADCC (titer range: 7.4–1721.6) and ADCP activities (titer range: 4–523.2). Notably, both ADCC and ADCP antibody titers positively correlated with the nAb titers in HCPs. In summary, we developed new tools for quantitative ADCC and ADCP analysis against SARS-CoV-2, which may facilitate further evaluations of Fc-mediated effector functions in preventing and treating against SARS-CoV-2.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Coelenterazine 55779-48-1 C26H21N3O3 252 suppliers $45.00-$14697.00
Coelenterazine 55779-48-1 C26H21N3O3 252 suppliers $45.00-$14697.00
Coelenterazine 55779-48-1 C26H21N3O3 252 suppliers $45.00-$14697.00
Coelenterazine 55779-48-1 C26H21N3O3 252 suppliers $45.00-$14697.00

Similar articles

IF:2.5

Development of an Indirect ELISA Based on the VP1 Protein for Detection of Antibodies against Water Buffalo Hunnivirus

Biochemical and biophysical research communications Qianyuan Liu, Xiaoying Feng,etc Published: 23 November 2024
IF:4.6

Peripheral neuropathy associated with monomethyl auristatin E-based antibody-drug conjugates

iScience Zhiwen Fu , Chen Gao ,etc Published: 20 October 2023
江川县| 全南县| 隆尧县| 垣曲县| 枣阳市| 奈曼旗| 华亭县| 漯河市| 连城县| 新和县| 灌云县| 普宁市| 巴塘县| 原平市| 禹州市| 龙门县| 米脂县| 孝义市| 宁德市| 遂昌县| 利川市| 大英县| 香格里拉县| 汕头市| 永和县| 普格县| 鱼台县| 竹北市| 衡水市| 五莲县| 梁山县| 萍乡市| 华容县| 宁国市| 哈巴河县| 大余县| 宜川县| 梁平县| 南安市| 南和县| 集安市|