一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Analytical Chemistry >>article
Analytical Chemistry

Analytical Chemistry

IF: 6.7
Download PDF

Click-Reaction-Triggered SERS Signals for Specific Detection of Monoamine Oxidase B Activity

Published:26 October 2020 DOI: 10.1021/acs.analchem.0c03017 PMID: 33103897
Xueqiang Wu, Yifang Li, Jinhua Wang, Haibo Zhou*, Xiao Tang, Ying Yang, Zhigang Wang, Dong Chen, Xia Zhou, Jialiang Guo, Huaihong Cai, Junxia Zheng*, Pinghua Sun*

Abstract

Human monoamine oxidases (MAOs) play important roles in maintaining the homeostasis of biogenic amines. One of its isoforms, monoamine oxidase B (MAOB), is thought to be involved in several neurodegenerative diseases, which make the selective detection of MAOB activity essential. In this work, a novel surface-enhanced Raman scattering (SERS) sensor was fabricated and the MAOB activity was specifically determined by detecting the SERS signals of an enzyme-catalyzed reaction product via an amine–aldehyde click reaction. This process was simply achieved by coating core–shell gold–silver nanoparticles ([email?protected] NPs) on 3-aminopropyl aminopropyl triethoxysilane (APTES)-modified glass, and then, a monolayer of cysteamine (CA) was attached to the nanoparticle surface as a linker through Ag–S bonds. Using phenethylamine (PA) as a specific substrate of MAOB, the enzyme product phenylacetaldehyde (PAA) will produce significant Raman signals via the amine–aldehyde click reaction with CA, while other molecules, such as MAOB and PA, have no signal output because they cannot form close interaction with nanoparticles due to the existence of a CA layer. This sensor was further used for the specific determination of MAOB activity in clinical blood samples and the MAOB inhibitor assessment successfully. Meanwhile, by changing the click reaction types and taking advantage of the SERS fingerprint peaks for the specific click reaction products, this strategy offers huge potential to detect multiple enzyme activities simultaneously and can be used for new click reaction screening, enzyme-related disease diagnosis, drug screening, and clinical diagnosis.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
NIALAMIDE 51-12-7 C16H18N4O2 63 suppliers $35.00-$2200.00
NIALAMIDE 51-12-7 C16H18N4O2 63 suppliers $35.00-$2200.00
NIALAMIDE 51-12-7 C16H18N4O2 63 suppliers $35.00-$2200.00
NIALAMIDE 51-12-7 C16H18N4O2 63 suppliers $35.00-$2200.00

Similar articles

察隅县| 济阳县| 邵阳市| 林口县| 宜兴市| 林口县| 玛曲县| 宝兴县| 黑河市| 罗定市| 包头市| 汕尾市| 正镶白旗| 安庆市| 昌江| 南陵县| 德保县| 岚皋县| 齐河县| 丹江口市| 邢台县| 巴彦淖尔市| 泰顺县| 丘北县| 通海县| 古丈县| 泰安市| 嘉荫县| 河北区| 呈贡县| 洛阳市| 扎兰屯市| 繁峙县| 特克斯县| 旅游| 尼玛县| 万全县| 佛学| 阳信县| 沙田区| 尼木县|