一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of Chromatography A >>article
Journal of Chromatography A

Journal of Chromatography A

IF: 3.8
Download PDF

An in-line method for high-throughput screening of protein tyrosine phosphatase receptor type O inhibitors by capillary electrophoresis based on electrophoretically mediated microanalysis

Published:17 November 2023 DOI: 10.1016/j.chroma.2023.464511
Xueting Qiu , Xuben Hou , Yue Yang , Hao Fang , Fei Cui , Xinying Yang

Abstract

Protein tyrosine phosphatase receptor type O (PTPRO) plays an important role in inflammation-related pathways and has become an emerging drug target. In this study, we developed an in-line capillary electrophoresis (CE) method for the investigation of the enzymatic activity of PTPRO, which was based on electrophoretically mediated microanalysis (EMMA). After a thorough method validation of the optimized conditions, this protocol was successfully employed to determine the kinetics of PTPRO as well as the half-maximal inhibitory concentration (IC50) of two typical PTPRO inhibitors. The final results were consistent with the values obtained through classical ultraviolet–visible (UV–vis) spectrophotometry. Our new method exhibited improved accuracy and reduced consumption, avoiding the disadvantages of traditional methods. This work provides a new strategy for PTPRO enzyme kinetic studies as well as inhibitory activity determination through capillary electrophoresis for the first time.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00

Similar articles

IF:2.3

Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening

Biochemistry and Biophysics Reports Avani Mehta , Prateek Raj ,etc Published: 1 July 2021
IF:2.5

Development of an Indirect ELISA Based on the VP1 Protein for Detection of Antibodies against Water Buffalo Hunnivirus

Biochemical and biophysical research communications Qianyuan Liu, Xiaoying Feng,etc Published: 23 November 2024
湘乡市| 大化| 丹阳市| 彭州市| 荆门市| 图木舒克市| 平舆县| 临江市| 如东县| 新丰县| 鲜城| 辛集市| 滦平县| 鄂伦春自治旗| 天等县| 金湖县| 昌宁县| 石阡县| 汕头市| 柳河县| 祁连县| 澄城县| 汝城县| 大洼县| 柞水县| 北碚区| 阳江市| 富阳市| 皮山县| 兴和县| 高州市| 嘉义市| 关岭| 临清市| 新田县| 泾源县| 浮梁县| 纳雍县| 育儿| 勃利县| 新田县|