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Journal of Chromatography A

Journal of Chromatography A

IF: 3.8
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An in-line method for high-throughput screening of protein tyrosine phosphatase receptor type O inhibitors by capillary electrophoresis based on electrophoretically mediated microanalysis

Published:17 November 2023 DOI: 10.1016/j.chroma.2023.464511
Xueting Qiu , Xuben Hou , Yue Yang , Hao Fang , Fei Cui , Xinying Yang

Abstract

Protein tyrosine phosphatase receptor type O (PTPRO) plays an important role in inflammation-related pathways and has become an emerging drug target. In this study, we developed an in-line capillary electrophoresis (CE) method for the investigation of the enzymatic activity of PTPRO, which was based on electrophoretically mediated microanalysis (EMMA). After a thorough method validation of the optimized conditions, this protocol was successfully employed to determine the kinetics of PTPRO as well as the half-maximal inhibitory concentration (IC50) of two typical PTPRO inhibitors. The final results were consistent with the values obtained through classical ultraviolet–visible (UV–vis) spectrophotometry. Our new method exhibited improved accuracy and reduced consumption, avoiding the disadvantages of traditional methods. This work provides a new strategy for PTPRO enzyme kinetic studies as well as inhibitory activity determination through capillary electrophoresis for the first time.

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Materials
Procduct Name CAS Molecular Formula Supplier Price
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00
Bis(maltolato)oxovanadium(IV) 38213-69-3 C12H10O7V 94 suppliers $9.00-$1947.00

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