一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of Chromatography A >>article
Journal of Chromatography A

Journal of Chromatography A

IF: 3.8
Download PDF

Detailed analysis of the effective and intra-particle diffusion coefficient of proteins at elevated pressure in columns packed with wide-pore core-shell particles

Published:27 November 2023 DOI: 10.1016/j.chroma.2023.464538
Leon E. Niezen , Tsukasa Sasaki , Donatela Sadriaj , Harald Ritchie , Ken Broeckhoven , Deirdre Cabooter , Gert Desmet

Abstract

To determine the efficiency that can be obtained in a packed-bed liquid-chromatography column for a particular analyte, a correct determination of the molecular and effective diffusion coefficients (Dm and Deff) of the analyte is required. The latter is usually obtained via peak parking experiments wherein the flow is stopped. As a result, the column pressure rapidly dissipates and the measurement is essentially conducted at ambient pressure. This is problematic for analytes whose retention depends on pressure, such as proteins and potentially other large (dipolar) molecules. In that case, a conventional peak parking experiment is expected to lead to large errors in Deff. To obtain a better estimate ofDeff, the present study reports on the use of a set-up enabling peak parking measurements under pressurized conditions. This approach allowed us to report, for the first time, Deff for proteins at elevated pressure under retained conditions. First, Deff was determined at a (average) pressure of bar for a set of proteins with varying size, namely: bradykinin, insulin, lysozyme, β-lactoglobulin, and carbonic anhydrase in a column packed with 400? core-shell particles. The obtained data were then compared to those of several small analytes: acetophenone, propiophenone, benzophenone, valerophenone, and hexanophenone. A clear trend between Deff and analyte size was observed. The set-up was then used to determine Deff of bradykinin and lysozyme at variable (average) pressures ranging from 28 bar to 430 bar. These experiments showed a decrease in intra-particle and surface diffusion with pressure, which was larger for lysozyme than bradykinin. The data show that pressurized peak parking experiments are vital to correctly determine Deff when the analyte retention varies significantly with pressure.

Substances (6)

Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
BRADYKININ 58-82-2 C50H73N15O11 121 suppliers $29.70-$1200.00
BRADYKININ 58-82-2 C50H73N15O11 121 suppliers $29.70-$1200.00
BRADYKININ 58-82-2 C50H73N15O11 121 suppliers $29.70-$1200.00
BRADYKININ 58-82-2 C50H73N15O11 121 suppliers $29.70-$1200.00

Similar articles

IF:0

Diffusion Coefficient of Tin(II) Methanesulfonate in Ionic Liquid and Methane Sulfonic Acid (MSA) Solvent

Metallurgical and Materials Transactions B Kok Kee Yang,?M. Mahmoudian,etc Published: 10 August 2011
IF:8.9

MoS2-based core-shell nanostructures: Highly efficient materials for energy storage and conversion applications

Journal of energy storage Pawanpreet Kour,?Deeksha,etc Published: 1 January 2023
IF:2.4

High-pressure polymorphism of cyclopentanol (C5H10O): the structure of cyclopentanol phase-V at 1.5 GPa.

Journal of Synchrotron Radiation S A Moggach, D R Allan,etc Published: 1 September 2005
福州市| 南安市| 无极县| 南充市| 桦川县| 临武县| 衡阳县| 荥阳市| 平湖市| 商城县| 锡林郭勒盟| 两当县| 红河县| 双桥区| 吴江市| 民丰县| 巴里| 焦作市| 石家庄市| 错那县| 巩留县| 沭阳县| 宝应县| 甘南县| 青冈县| 巴彦淖尔市| 宣恩县| 临泉县| 武川县| 娱乐| 云林县| 巧家县| 雷州市| 祁东县| 沙田区| 大冶市| 高阳县| 岳阳县| 宽城| 东辽县| 元谋县|