一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Scientific Reports >>article
Scientific Reports

Scientific Reports

IF: 3.8
Download PDF

Exploring the mechanism and phytochemicals in Psoraleae Fructus-induced hepatotoxicity based on RNA-seq, in vitro screening and molecular docking

Published:19 January 2024 DOI: 10.1038/s41598-023-50454-0
Huiying Shang, Xian Liu, Jinchao Pan, Hongbo Cheng, Zengchun Ma, Chengrong Xiao, Yue Gao

Abstract

Psoraleae Fructus (PF) is a widely-used herb with diverse pharmacological activities, while its related hepatic injuries have aroused public concerns. In this work, a systematic approach based on RNA sequencing (RNA-seq), high-content screening (HCS) and molecular docking was developed to investigate the potential mechanism and identify major phytochemicals contributed to PF-induced hepatotoxicity. Animal experiments proved oral administration of PF water extracts disturbed lipid metabolism and promoted hepatic injuries by suppressing fatty acid and cholesterol catabolism. RNA-seq combined with KEGG enrichment analysis identified mitochondrial oxidative phosphorylation (OXPHOS) as the potential key pathway. Further experiments validated PF caused mitochondrial structure damage, mtDNA depletion and inhibited expressions of genes engaged in OXPHOS. By detecting mitochondrial membrane potential and mitochondrial superoxide, HCS identified bavachin, isobavachalcone, bakuchiol and psoralidin as most potent mitotoxic compounds in PF. Moreover, molecular docking confirmed the potential binding patterns and strong binding affinity of the critical compounds with mitochondrial respiratory complex. This study unveiled the underlying mechanism and phytochemicals in PF-induced liver injuries from the view of mitochondrial dysfunction.

Substances (13)

Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
Corylin 53947-92-5 C20H16O4 189 suppliers $30.00-$1400.00
Corylin 53947-92-5 C20H16O4 189 suppliers $30.00-$1400.00
Corylin 53947-92-5 C20H16O4 189 suppliers $30.00-$1400.00
Corylin 53947-92-5 C20H16O4 189 suppliers $30.00-$1400.00

Similar articles

IF:6.8

The ways for ginsenoside Rh2 to fight against cancer: the molecular evidences in?vitro and in?vivo.

Journal of Ginseng Research Qi-rui Hu , Yao Pan ,etc Published: 1 March 2023
IF:2.3

Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening

Biochemistry and Biophysics Reports Avani Mehta , Prateek Raj ,etc Published: 1 July 2021
IF:0

Exploring the effect of capsaicin on gene expression and chemotherapy sensitization in gastric cancer cells

bioRxiv - Cancer Biology Weijian Meng, Sophia Xie,etc Published: 7 September 2024
兴城市| 六安市| 盈江县| 铁力市| 临朐县| 中宁县| 芦山县| 双辽市| 岚皋县| 平武县| 奉贤区| 中方县| 哈巴河县| 八宿县| 禹州市| 措美县| 徐汇区| 潍坊市| 泾川县| 搜索| 桐乡市| 麻城市| 登封市| 凤凰县| 阿克陶县| 涞水县| 乌审旗| 阿拉尔市| 灌南县| 广平县| 香港 | 禄劝| 高尔夫| 巍山| 石渠县| 莱西市| 肇东市| 白水县| 商河县| 遂宁市| 岗巴县|