一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of Chromatography A >>article
Journal of Chromatography A

Journal of Chromatography A

IF: 3.8
Download PDF

Modeling multi-component separation in hydrophobic interaction chromatography with improved parameter-by-parameter estimation method

Published:29 June 2024 DOI: 10.1016/j.chroma.2024.465121 PMID: 38959659
Yu-Xiang Yang , Zhi-Yuan Lin , Yu-Cheng Chen , Shan-Jing Yao , Dong-Qiang Lin

Abstract

Mechanistic models are powerful tools for chromatographic process development and optimization. However, hydrophobic interaction chromatography (HIC) mechanistic models lack an effective and logical parameter estimation method, especially for multi-component system. In this study, a parameter-by-parameter method for multi-component system (called as mPbP-HIC) was derived based on the retention mechanism to estimate the six parameters of the Mollerup isotherm for HIC. The linear parameters (ks,i and keq,i) and nonlinear parameters (ni and qmax,i) of the isotherm can be estimated by the linear regression (LR) and the linear approximation (LA) steps, respectively. The remaining two parameters (kp,i and kkin,i) are obtained by the inverse method (IM). The proposed method was verified with a two-component model system. The results showed that the model could accurately predict the protein elution at a loading of 10 g/L. However, the elution curve fitting was unsatisfactory for high loadings (12 g/L and 14 g/L), which is mainly attributed to the demanding experimental conditions of the LA step and the potential large estimation error of the parameter qmax. Therefore, the inverse method was introduced to further calibrate the parameter qmax, thereby reducing the estimation error and improving the curve fitting. Moreover, the simplified linear approximation (SLA) was proposed by reasonable assumption, which provides the initial guess of qmax without solving any complex matrix and avoids the problem of matrix unsolvable. In the improved mPbP-HIC method, qmax would be initialized by the SLA and finally determined by the inverse method, and this strategy was named as SLA+IM. The experimental validation showed that the improved mPbP-HIC method has a better curve fitting, and the use of SLA+IM reduces the error accumulation effect. In process optimization, the parameters estimated by the improved mPbP-HIC method provided the model with excellent predictive ability and reasonable extrapolation. In conclusion, the SLA+IM strategy makes the improved mPbP-HIC method more rational and can be easily applied to the practical separation of protein mixture, which would accelerate the process development for HIC in downstream of biopharmaceuticals.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
LYSOZYME 9001-63-2 C11H20NO6- 202 suppliers $48.00-$1008.00
LYSOZYME 9001-63-2 C11H20NO6- 202 suppliers $48.00-$1008.00
LYSOZYME 9001-63-2 C11H20NO6- 202 suppliers $48.00-$1008.00
LYSOZYME 9001-63-2 C11H20NO6- 202 suppliers $48.00-$1008.00

Similar articles

IF:0

Considering parameter uncertainties in the design of safe processes

Computer-aided chemical engineering M. Shacham, N. Brauner,etc Published: 1 January 1900
IF:3.1

RP-HPLC method for simultaneous estimation of bisoprolol fumarate and hydrochlorothiazide in tablet formulation

Journal of pharmaceutical and biomedical analysis Sneha J. Joshi , Pradnya A. Karbhari ,etc Published: 8 July 2010
黄梅县| 南溪县| 重庆市| 华安县| 绿春县| 赤水市| 日照市| 罗田县| 东源县| 丽江市| 平遥县| 教育| 东丽区| 澄迈县| 安康市| 郓城县| 云和县| 商南县| 双桥区| 崇义县| 烟台市| 阿鲁科尔沁旗| 拉萨市| 丹棱县| 巨野县| 织金县| 兴隆县| 安顺市| 沙田区| 无为县| 惠东县| 剑阁县| 洱源县| 汉沽区| 周口市| 贵州省| 甘洛县| 富宁县| 泌阳县| 固始县| 华安县|