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International Journal of Biological Macromolecules

International Journal of Biological Macromolecules

IF: 7.7
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Critical roles of small silk fibroin molecules in the self-assembly and properties of regenerated silk fibroin

Published:21 November 2024 DOI: 10.1016/j.ijbiomac.2024.137926 PMID: 39577537
Hao Li, Yuanyuan Guo, Chun Liu

Abstract

Silk is primarily composed of silk fibroin (SF) and silk sericin (SS), with SF significantly contributing to the mechanical properties of silk fibers. SF consists of the large molecular fibroin heavy chain (Fib-LH), small molecular fibroin light chain (Fib-L), and P25 protein. Degumming is a crucial step in both the silk reeling process and the preparation of regenerated silk fibroin (RSF), but it can cause damage to Fib-LH. This study investigates how degumming affects small SF molecules and their influence on the properties of silk fibers and RSF. A gradient degumming treatment using various reagents was employed. SS antibody detection indicated that 3?g/L papain and sodium carbonate (Na2CO3) at concentrations ≥0.2?% almost completely removed SS. SF antibody detection revealed that Na2CO3 degumming severely damaged Fib-H and degraded Fib-L and P25. While tensile tests showed that this damage did not significantly affect the mechanical properties of SF fibers, the loss of small SF molecules reduced the mechanical properties of the RSF membranes and delayed RSF gelation. Atomic force microscopy demonstrated that RSF containing Fib-H of similar molecular weight (100–180?kDa) can self-assemble into nanofibrils when small SF molecules are present, whereas 0.5?% Na2CO3-degummed RSF lacking these small SF molecules cannot form nanofibrils. By adding additional small SF molecules to the 0.5?% Na2CO3-degummed RSF, nanofibrils can be formed. This research highlights the critical role of small SF molecules in the properties of RSF and provides a theoretical foundation for the development of RSF-derived materials.

Substances (3)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Sodium carbonate 497-19-8 CH2O3.2Na 1713 suppliers $14.00-$5120.00
Calcium chloride 10043-52-4 CaCl2 1213 suppliers $5.00-$26371.00
Papain 9001-73-4 C9H14N4O3 679 suppliers $27.00-$3650.00

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