一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Stem cell research >>article
Stem cell research

Stem cell research

IF: 0.8
Download PDF

Establishment of a CPAMD8-GFP reporter human embryonic stem cell line, IBBDe001-B, using CRISPR/Cas9 editing

Published:22 November 2024 DOI: 10.1016/j.scr.2024.103615
Shun Zhang, Jiahang Li, Shasha Li

Abstract

The human CPAMD8 gene encodes proteins in the A2M/C3 (alpha-2-macroglobulin/complement 3) family, predominantly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body. Mutations in CPAMD8 have been linked to anterior segment dysplasia and congenital glaucoma. Using CRISPR/Cas9 editing, we inserted a 3*EAAAK-EGFP fluorescent tag into the CPAMD8 gene, enabling real-time observation of its expression and providing insights into its biological functions. The resulting gene-edited cell line retained normal stem cell morphology and karyotype, expressed essential pluripotency markers, and exhibited differentiation potential.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Ethylenediaminetetraacetic acid 60-00-4 C10H16N2O8 1439 suppliers $6.00-$6397.62
Triton X-100 9002-93-1 (C2H4O)nC14H22O 844 suppliers $16.00-$5950.00
4',6-Diamidino-2-phenylindole dihydrochloride 28718-90-3 C16H16ClN5 610 suppliers $15.00-$4800.00
Y-27632 DIHYDROCHLORIDE 146986-50-7 C14H21N3O 277 suppliers $16.00-$2691.10

Similar articles

IF:5.3

A preliminary study: the anti-proliferation effect of salidroside on different human cancer cell lines.

Cell Biology and Toxicology Xiaolan Hu, Shuxin Lin,etc Published: 1 December 2010
阿克苏市| 曲水县| 方城县| 武强县| 隆回县| 赫章县| 德格县| 峡江县| 武强县| 如皋市| 克东县| 安龙县| 鸡西市| 玉溪市| 安图县| 商河县| 左贡县| 寻甸| 雷州市| 林口县| 新建县| 湘乡市| 罗江县| 铜梁县| 青神县| 嘉峪关市| 大兴区| 思南县| 红原县| 淮安市| 绍兴县| 抚宁县| 呈贡县| 拉孜县| 张家港市| 喀喇沁旗| 封丘县| 栾城县| 邓州市| 敦煌市| 杭锦旗|