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Journal of Extracellular Vesicles

Journal of Extracellular Vesicles

IF: 15.5
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Glycoengineered extracellular vesicles released from antibacterial hydrogel facilitate diabetic wound healing by promoting angiogenesis

Published:27 November 2024 DOI: 10.1002/jev2.70013 PMID: 39600241
Kewei Wang, Qin He, Mengmeng Yang, Qincheng Qiao, Jun Chen, Jia Song, Nan Zang, Huiqing Hu, Longqing Xia, Yingyue Xiang, Fei Yan, Xinguo Hou, Li Chen

Abstract

Diabetic wounds have become a global healthcare burden owing to impaired angiogenesis and persistent infections. Extracellular vesicles (EVs) can improve diabetic wounds, though their targeting ability is limited. In this study, we investigated the performance of a novel hydrogel dressing comprised of gelatin methacryloyl, glycoengineered EVs, and polylysine in treating infected diabetic wounds. High-throughput single-cell RNA sequencing (scRNA-seq) and immunofluorescence staining revealed that E-selectin (SELE) levels were higher in diabetic wounds than in non-diabetic wounds. Mesenchymal stromal cells (MSCs) were transfected with a lentivirus containing fucosyltransferase VII (FUT7) and a CD63-P19-Nluc vector to enhance the expression of sialyl Lewis X (sLeX), the ligand of E-selectin, on the surface of EVs (s-EVs) derived from transfected MSCs (s-MSCs). s-EVs can target human umbilical vein endothelial cells (HUVECs) under lipopolysaccharide stimulation and promote the function of stimulated HUVECs in vitro. To promote and sustain the release of s-EVs, we fabricated a gelatin methacryloyl (Gel)/poly-L-lysine methacryloyl (PL)-5 hydrogel with good antibacterial ability, biocompatibility and mechanical properties. In a mouse experiment, s-EV@Gel/PL-5 exhibited excellent angiogenesis and anti-inflammatory abilities and further promoted the healing of infected diabetic wounds. Our findings demonstrated the potential of the s-EV@Gel/PL-5 hydrogel in the clinical treatment of diabetic infectious wounds.

Substances (2)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Streptozotocin 18883-66-4 C8H15N3O7 642 suppliers $17.00-$3712.25
PUROMYCIN 53-79-2 C22H29N7O5 147 suppliers $39.00-$1200.00

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