一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Phytochemistry >>article
Phytochemistry

Phytochemistry

IF: 3.2
Download PDF

Targeted isolation of lignans and triterpenoids from kadsura coccinea by molecular networking and anti-RA-FLS activity

Published:27 November 2024 DOI: 10.1016/j.phytochem.2024.114341 PMID: 39613277
Shi-qi Liu, Qing-ling Xie, Yu-pei Yang

Abstract

In this study, six compounds (four triterpenoids named heilaohutriterpenes A-D and two lignans name heilaohusuins F and G) together with 21 known compounds were isolated from roots of Kadsura coccinea (Lem.) A. C. Smith guided by molecular networking. Their structures were determined using a combination of HR-ESI-MS, 1D, 2D-NMR anatysis, NMR calculation, and electronic circular dichroism (ECD) calculations. Moreover, the ability of the isolated compounds to inhibit the proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells was evaluated in vitro. Heilaohutriterpene B (2), heilaohutriterpene D (4), coccinone B (7), and kadsuralignan H (24) demonstrated significant inhibitory activities against RA-FLS cells, with IC50 values of 9.57 ± 0.84, 16.22 ± 1.71, 3.08 ± 1.59, and 19.09 ± 2.42 μM, respectively. Meanwhile, western blotting analysis revealed that compound 2 down-regulated the level of P-NF-κB p65 and up-regulated that of Bax and IκBα. These results collectively suggest that compound 2 promoted the apoptosis of RA-FLS cells by inhibiting the NF-κB pathway. Taken together, this study contributed to the structural diversity of compounds derived from K. coccinea and lays a basis for further anti-RA-related studies.

Substances (2)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Methotrexate 59-05-2 C20H22N8O5 812 suppliers $8.00-$12270.00
Chloroform-d 865-49-6 CCl3D 270 suppliers $15.00-$1310.00

Similar articles

老河口市| 龙川县| 拉萨市| 土默特左旗| 江源县| 达州市| 耿马| 自治县| 荆州市| 加查县| 沙湾县| 康乐县| 成安县| 轮台县| 视频| 申扎县| 孟州市| 天津市| 萨迦县| 常山县| 冀州市| 湘潭市| 西盟| 张掖市| 年辖:市辖区| 尼勒克县| 尼勒克县| 邯郸市| 连城县| 广汉市| 阿瓦提县| 彭水| 嵩明县| 东宁县| 丘北县| 盘山县| 绥棱县| 罗源县| 泗阳县| 平湖市| 故城县|