一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Stem Cell Research & Therapy >>article
Stem Cell Research & Therapy

Stem Cell Research & Therapy

IF: 7.1
Download PDF

CX43-mediated mitochondrial transfer maintains stemness of KG-1a leukemia stem cells through metabolic remodeling

Published:2 December 2024 DOI: 10.1186/s13287-024-04079-3 PMID: 39623456
Huihui Fu,?Xiaoqing Xie,?Liuyue Zhai,?Yi Liu,?Yifeng Tang,?Sanxiu He,?Jun Li,?Qing Xiao,?Guofa Xu,?Zailin Yang,?Xiaomei Zhang,?Yao Liu

Abstract

Background: Acute myeloid leukemia (AML) is characterized by abundant immature myeloid cells, relapse and refractory due to leukemia stem cells (LSCs). Bone marrow mesenchymal stem/ stromal cells (BMSCs) supported LSCs survival, meanwhile, chemotherapy improved connexin43 (CX43) expression. CX43, as the most intercellular gap junction, facilitated transmit mitochondria from BMSCs into AML. We hypothesized that increased mitochondria transferred from BMSCs supported metabolic remodeling in LSCs to sustain their stemness.

Methods: Primary BMSCs from AML patients were isolated. CX43-BMSCs, overexpressing CX43, were cocultured with KG-1a cells. Fluorescence and confocal microscopy observed mitochondrial transfer. Flow cytometry, EdU assay, and clonogenicity evaluated cell cycle, proliferation, and clonogenic potential. Xenograft mouse models were used to evaluate the tumorigenicity of KG-1a in vivo. Seahorse, RNA-seq, and LC-MS assessed mitochondrial function, transcriptomes, and metabolites post-coculture.

Results: CX43-BMSCs promoted unidirectional mitochondrial transfer, enhancing KG-1a adhesion and proliferation to maintain LSCs stemness in vitro and vivo. RNA-seq revealed coculture with CX43-BMSCs upregulated genes related to adhesion, proliferation, and migration in KG-1a cells. Elevated CX43 expression strengthened BMSCs-KG-1a interaction, facilitating mitochondrial transfer and nucleoside metabolism, fueling KG-1a cells. This enhanced mitochondrial energy metabolism, promoting metabolic reprogramming and clonogenicity.

Conclusion: CX43-mediated mitochondrial transfer from BMSCs to KG-1a enhances LSCs adhesion, proliferation, clonogenicity, and metabolic reprogramming. CX43 emerges as a potential therapeutic target for AML by sustaining LSCs stemness through metabolic remodeling.

Substances (5)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Methyl cellulose 9004-67-5 C20H38O11 629 suppliers $4.00-$2550.00
Busulfan 55-98-1 C6H14O6S2 427 suppliers $6.00-$444.00
7-AMINOACTINOMYCIN D 7240-37-1 C62H87N13O16 216 suppliers $60.00-$979.00
PUROMYCIN 53-79-2 C22H29N7O5 146 suppliers $39.00-$1200.00
DAPI 47165-04-8 C16H15N5 16 suppliers $504.53-$1715.00

Similar articles

IF:10

Localized Hydrogel Microspheres for Osteoarthritis Treatment: Recruitment and Differentiation of Stem Cells

Advanced Healthcare Materials Qiming Pang, Zhuolin Chen,etc Published: 28 November 2024
IF:7.1

Itaconic acid ameliorates necrotizing enterocolitis through the TFEB-mediated autophagy-lysosomal pathway

Free Radical Biology and Medicine Baozhu Chen, Yufeng Liu,etc Published: 19 November 2024
永新县| 饶阳县| 宜黄县| 奉化市| 通州区| 新晃| 雷山县| 溧阳市| 普洱| 临清市| 股票| 扶绥县| 东辽县| 平泉县| 万山特区| 达拉特旗| 商南县| 临洮县| 嵊泗县| 库车县| 苏尼特右旗| 乳源| 收藏| 保德县| 朝阳县| 德州市| 舞阳县| 上林县| 利川市| 峨眉山市| 姚安县| 东乌| 唐海县| 民权县| 开封县| 张家界市| 赤城县| 太原市| 张家川| 布拖县| 来宾市|