一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Chemical Engineering Journal >>article
Chemical Engineering Journal

Chemical Engineering Journal

IF: 13.3
Download PDF

A portable microfluidic platform for ultra-sensitive detection of ovarian cancer biomarkers via cascade signal amplification

Published:4 December 2024 DOI: 10.1016/j.cej.2024.158289
Yuzhen Wang, Wenxiu Long, Yanping Wang, Yuanyue Han, Fei Zeng, Jingjing Li, Bangshun He, Yujun Song, Yanfeng Gao

Abstract

Early detection of ovarian cancer is crucial for improving treatment outcomes and increasing survival rates, as this malignancy is often diagnosed at an advanced stage due to the lack of specific early symptoms. In this study, we present a novel diagnostic platform integrating platinum nanozyme-induced cascade signal amplification with a gas-driven visual amplification chip (VAC) for the sensitive and specific detection of the ovarian cancer biomarkers CA125 and HE4. This system leverages a sandwich complex formed by capture antibody-functionalized magnetic silica nanoparticles (Ab1-MSNPs) and detection antibody-functionalized platinum nanoparticles (Ab2-PtNPs) in the presence of target antigens. The PtNPs’ peroxidase-like activity catalyzes dopamine polymerization, leading to polydopamine (PDA) deposition and subsequent capture of 4-mercaptophenylboronic acid-modified PtNPs, amplifying the detection signal. The VAC achieved exceptional sensitivity, with detection limits as low as 0.001?U?mL?1 for CA125 and 0.1?pg?mL?1 for HE4, surpassing traditional methods. Finally, the platform demonstrated a strong correlation with electrochemiluminescence assays, making it a promising tool for point-of-care testing and early ovarian cancer detection in clinical settings.

Substances (1)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Ethyl silicate 78-10-4 C8H20O4Si 505 suppliers $12.00-$3300.00

Similar articles

东乌珠穆沁旗| 金阳县| 东明县| 淳安县| 吉首市| 宣威市| 江陵县| 大城县| 理塘县| 临江市| 芦山县| 乡宁县| 威海市| 托里县| 开江县| 哈巴河县| 清镇市| 平乡县| 九江市| 精河县| 尉氏县| 肇东市| 高安市| 望江县| 沂水县| 开阳县| 霍山县| 湖州市| 沽源县| 高安市| 东光县| 定兴县| 富民县| 新乡市| 肃宁县| 亚东县| 密云县| 海南省| 徐水县| 临清市| 威宁|