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Talanta

Talanta

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Target-driven Functionalized DNA Hydrogel Capillary Sensor for SARS-CoV-2 Dual-mode Detection

Published:4 December 2024 DOI: 10.1016/j.talanta.2024.127342 PMID: 39644672
Jing-Bo Jiao,?Qing Kang,?Shu-Xin Cui,?Jiang-Li Cao,?Tong Lin,?Chen-Jing Ma,?Ze-Hui Xiao,?Ting Du,?Nan Wang,?Xin-Jun Du,?Shuo Wang

Abstract

Coronavirus disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused secondary pandemic, which still poses a serious threat to physical health and economic development. Herein, the target-driven functionalized DNA hydrogel capillary sensor based on cascade signal amplification and carbon coated cobalt manganese modified by prussian blue and platinum nanoparticles (MnCo@C-Pt-PB NPs) has been successfully developed for dual-mode detection of SARS-CoV-2. The cascade signal amplification triggered by target RNA causes the permeability of the DNA hydrogel loaded in the capillary to be destroyed, thereby releasing the embedded MnCo@C-Pt-PB NPs as signal molecules into 3,3',5,5'-tetramethylbenzidine/hydrogen peroxide (TMB/H2O2) solution under the driving of capillarity. The colorless TMB is then catalyzed to blue oxidation products (oxTMB) due to peroxidase-like activity of MnCo@C-Pt-PB NPs, and MnCo@C-Pt-PB NPs and oxTMB with photothermal properties synergistically increase the system temperature under near-infrared irradiation, which are recorded by portable devices to achieve dual-mode detection. Signals intensity are proportional to the logarithm of T-RNA concentration in a wide detection range (100 aM-100 pM), with a detection limit of 100 aM. Moreover, the reliability of the developed method in oropharyngeal swabs samples has also been validated. The signal conversion and amplification function of functionalized DNA hydrogel enhances the convenience, sensitivity and versatility of the developed method, which is promising to be applied in environmental safety, molecular diagnostic assays and disease prevention.

Substances (6)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Ascorbic Acid 50-81-7 C6H8O6 1968 suppliers $6.00-$2380.00
Potassium chloride 7447-40-7 KCl 1593 suppliers $6.00-$12480.00
Tris(hydroxymethyl)aminomethane 77-86-1 C4H11NO3 1524 suppliers $5.00-$8920.00
Ammonium persulfate 7727-54-0 H8N2O8S2 767 suppliers $10.16-$3100.00
Acrylamide 79-06-1 C3H5NO 692 suppliers $12.70-$1772.00
5,5-DIMETHYL-1-PYRROLINE N-OXIDE 3317-61-1 C6H11NO 285 suppliers $9.00-$1720.00
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