一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Macromolecules >>article
Macromolecules

Macromolecules

IF: 5.09
Download PDF

Enhancing the Electron Radiation Resistance of Macromolecules in Cryo-TEM Imaging Using Sodium Ascorbate

Published:10 December 2024 DOI: 10.1021/acs.macromol.4c02108
Pilan Zhang, Xiaoduo Dong, Haiqin Du, Shiwen Cui, Jingyi Liu, Yixiang He, Wenjie Ren, Hui Wang, Ying Zhang, Yifei Xu

Abstract

Electron radiation damage hinders the high-resolution cryogenic transmission electron microscopy (cryo-TEM) imaging of macromolecules. In this work, sodium ascorbate (SA) was used to eliminate reactive oxygen species generated during electron radiation, thereby enhancing the electron radiation resistance of macromolecular samples in cryo-TEM experiments. The effectiveness of SA on protecting sample morphology and structure was investigated for representative types of macromolecular samples, including collagen, cellulose, liposome, and apoferritin, and compared with other radical scavengers. Our findings revealed that SA provided the most effective protection for collagen, increasing its dose limit by more than 40%. Similar effects were observed for cellulose and apoferritin. Importantly, the addition of SA does not change the three-dimensional structure of apoferritin, as confirmed by a 2.2 ? resolution single particle analysis, further indicating the practicality of the method.

Substances (3)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Glycerol 56-81-5 C3H8O3 2251 suppliers $5.00-$6910.00
Sodium chloride 7647-14-5 ClNa 1692 suppliers $5.00-$6678.62
Cholesterol 57-88-5 C27H46O 1167 suppliers $10.00-$71130.00

Similar articles

IF:14.3

Enhancing the Therapeutic Potential of Peptide Antibiotics Using Bacteriophage Mimicry Strategies

Advanced Science Hongping Wan, Xinyi Zhong,etc Published: 25 November 2024
IF:0

ENHANCING THE SUNSCREEN EFFICACY OF BEMOTRIZINOL MICROPIGMENT BY USING O/W NANOEMULSION TOPICAL PREPARATIONS

International Journal of Pharmacy and Pharmaceutical Sciences Dina Hashim,?N. M. Sheta,etc Published: 17 May 2019
IF:3.6

Ascorbate as a biosynthetic precursor in plants.

Annals of botany Seth Debolt, Vanessa Melino,etc Published: 1 January 2007
楚雄市| 赞皇县| 泸西县| 桦甸市| 永福县| 海门市| 穆棱市| 定结县| 富宁县| 浦城县| 恩平市| 永川市| 抚宁县| 瑞安市| 武宁县| 防城港市| 宁津县| 石屏县| 邮箱| 宿松县| 临邑县| 正镶白旗| 萍乡市| 东海县| 柳林县| 巍山| 大庆市| 万荣县| 榕江县| 民勤县| 镇巴县| 凤冈县| 漳州市| 格尔木市| 城口县| 抚远县| 肃南| 临桂县| 桓仁| 临邑县| 铜梁县|