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Talanta

Talanta

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A graphene-based fluorescent nanoprobe for simultaneous imaging of dual miRNAs in living cells

Published:1 April 2021 DOI: 10.1016/j.talanta.2020.121947 PMID: 33592702
Xiang Xiong , Wenya Dang , Ruxin Luo , Ying Long , Chunyi Tong , Liqin Yuan , Bin Liu

Abstract

MicroRNAs (miRNAs) are regarded as important biomarkers for disease diagnostics and therapeutics due to their significant regulatory roles in physiologic and pathologic processes. Herein, a versatile nanoprobe based on reduced graphene oxide (rGO) and nucleic acid (DNA) probe was prepared for simultaneously visualize miR-451a and miR-214–3p in living cells. In vitro experiments demonstrated that the nanoprobe exhibits excellent selectivity and outstanding sensitivity as low as 1?nM towards miR-451a and miR-214–3p. Moreover, the detection signals of miRNAs have good linearity in their respective concentration ranges (miR-451a: 1–100?nM, Y1?=?9.3062X1+114.85 (R2?=?0.9965). miR-214–3p: 1–200?nM, Y2?=?1.4424X2+91.312 (R2?=?0.9961)). Finally, simultaneous dual-color imaging of miR-451a and miR-214–3p in human breast cancer cells (MDA-MB-231) was realized by exploiting the P1&P2@rGO nanoprobe. In summary, this simple and effective strategy provides a general sensing platform for highly sensitive detection and simultaneous imaging of dual miRNAs in living cells.

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