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Journal of Chromatography B

Journal of Chromatography B

IF: 2.8
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Establishment of LC-MS/MS method for determination of aloperine in rat plasma and its application in preclinical pharmacokinetics

Published:30 May 2021 DOI: 10.1016/j.jchromb.2021.122671 PMID: 33819795
Shengbo Huang , Yuanjin Zhang , Yanfang Zhang , Jie Liu , Zongjun Liu , Xin Wang

Abstract

Aloperine, a novel natural active alkaloid derived from Sophora alopecuroides L., has attracted much attention for its anti-inflammatory, antiviral, anti-tumor, anti-allergy and other pharmacological activities. In this study, we first established and validated an efficient and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of aloperine in rat plasma. Cytisine was used as the internal standard (IS). The separation of aloperine and IS was conducted on a Phenomenex Luna Omega Polar C18 column (2.1?×?50?mm, 1.6?μm) with 0.3% (v/v) formic acid aqueous (containing 5?mM ammonium acetate) and 0.3% (v/v) formic acid acetonitrile using isocratic elution condition at a flow rate of 0.20?mL/min. Aloperine and IS were determined under the transitions of m/z 233.2?→?98.1 and m/z 191.2?→?148.2 (positive ionization mode), respectively. The calibration curve of aloperine was established in the range of 5 (LLOQ) to 2000?ng/mL (r2?=?0.994). The well validated method was full compliance with the bioanalytical method validation of FDA, and was applied to the pharmacokinetic study of aloperine in Sprague-Dawley rats after 50?mg/kg oral administration and 5?mg/kg intravenous injection. This study provides valuable references for the further study of Sophora alopecuroides L., especially for the drug development and clinical application of aloperine.

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Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
CYTISINE 485-35-8 C11H14N2O 503 suppliers $5.00-$1900.00
CYTISINE 485-35-8 C11H14N2O 503 suppliers $5.00-$1900.00
CYTISINE 485-35-8 C11H14N2O 503 suppliers $5.00-$1900.00
CYTISINE 485-35-8 C11H14N2O 503 suppliers $5.00-$1900.00
ALOPERINE 56293-29-9 C15H24N2 206 suppliers $33.00-$750.00
ALOPERINE 56293-29-9 C15H24N2 206 suppliers $33.00-$750.00
ALOPERINE 56293-29-9 C15H24N2 206 suppliers $33.00-$750.00
ALOPERINE 56293-29-9 C15H24N2 206 suppliers $33.00-$750.00

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