一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Cancer Cell International >>article
Cancer Cell International

Cancer Cell International

IF: 5.3
Download PDF

Etoposide induces cell death via mitochondrial-dependent actions of p53.

Published:7 August 2015 DOI: 10.1186/s12935-015-0231-z PMID: 26251638
Sarwat Jamil, Irene Lam, Maryam Majd, Shu-Huei Tsai, Vincent Duronio

Abstract

Background: Etoposide has been used clinically in cancer treatment, as well as in numerous research studies, for many years. However, there is incomplete information about its exact mechanism of action in induction of cell death.

Methods: Etoposide was compared at various concentrations to characterize the mechanisms by which it induces cell death. We investigated its effects on mouse embryonic fibroblasts (MEFs) and focused on both transcriptional and non-transcriptional responses of p53.

Results: Here we demonstrate that treatment of MEFs with higher concentrations of etoposide induce apoptosis and activate the transcription-dependent functions of p53. Interestingly, lower concentrations of etoposide also induced apoptosis, but without any evidence of p53-dependent transcription up-regulation. Treatment of MEFs with an inhibitor of p53, Pifithrin-α, blocked p53-dependent transcription but failed to rescue the cells from etoposide-induced apoptosis. Treatment with PES, which inhibits the mitochondrial arm of the p53 pathway inhibited etoposide-induced cell death at all concentrations tested.

Conclusions: We have demonstrated that transcriptional functions of p53 are dispensable for etoposide-induced cell death. The more recently characterized effects of p53 at the mitochondria, likely involving its interactions with BCL-2 family members, are thus more important for etoposide's actions.

Substances (3)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Etoposide 33419-42-0 C29H32O13 693 suppliers $9.00-$17300.00
Pifithrin-α (PFTα) 63208-82-2 C16H19BrN2OS 188 suppliers $20.00-$3108.00
PES 7 suppliers Inquiry

Similar articles

IF:5.3

The Mitochondrial-Derived Peptide MOTS-c Alleviates Radiation Pneumonitis via an Nrf2-Dependent Mechanism

ACS Applied Nano Materials Yanli Zhang, Jianfeng Huang,etc Published: 17 May 2024
IF:2.6

Ammonium lauryl sulfate-induced apoptotic cell death may be due to mitochondrial dysfunction triggered by caveolin-1

Toxicology in Vitro Eun-Jung Park , Eunsol Seong ,etc Published: 1 June 2019
IF:0

Phosphatidylserine transport in cell life and death

arXiv - QuanBio - Subcellular Processes Alenka {?}opi{?}CRBM, Thibaud DieudonnéI2BC,etc Published: 9 November 2023
渝中区| 西昌市| 彭山县| 绍兴市| 绍兴市| 子长县| 无极县| 闻喜县| 油尖旺区| 承德市| 油尖旺区| 株洲市| 和龙市| 治多县| 济阳县| 墨脱县| 余干县| 顺平县| 郧西县| 光山县| 梁平县| 延长县| 大方县| 保定市| 米林县| 眉山市| 常州市| 五莲县| 湖南省| 利川市| 广汉市| 吉安市| 开江县| 壶关县| 宁安市| 吕梁市| 葵青区| 乌鲁木齐市| 松滋市| 清水县| 罗平县|