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Metabolic engineering

Metabolic engineering

IF: 6.79
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Construction of a Corynebacterium glutamicum platform strain for the production of stilbenes and (2S)-flavanones

Published:1 November 2016 DOI: 10.1016/j.ymben.2016.06.003 PMID: 27288926
Nicolai Kallscheuer, Michael Vogt, Anton Stenzel, Jochem G?tgens, Michael Bott, Jan Marienhagen

Abstract

Corynebacterium glutamicum is an important organism in industrial biotechnology for the microbial production of bulk chemicals, in particular amino acids. However, until now activity of a complex catabolic network for the degradation of aromatic compounds averted application of C. glutamicum as production host for aromatic compounds of pharmaceutical or biotechnological interest. In the course of the construction of a suitable C. glutamicum platform strain for plant polyphenol production, four gene clusters comprising 21 genes involved in the catabolism of aromatic compounds were deleted. Expression of plant-derived and codon-optimized genes coding for a chalcone synthase (CHS) and a chalcone isomerase (CHI) in this strain background enabled formation of 35?mg/L naringenin and 37?mg/L eriodictyol from the supplemented phenylpropanoids p-coumaric acid and caffeic acid, respectively. Furthermore, expression of genes coding for a 4-coumarate: CoA-ligase (4CL) and a stilbene synthase (STS) led to the production of the stilbenes pinosylvin, resveratrol and piceatannol starting from supplemented phenylpropanoids cinnamic acid, p-coumaric acid and caffeic acid, respectively. Stilbene concentrations of up to 158?mg/L could be achieved. Additional engineering of the amino acid metabolism for an optimal connection to the synthetic plant polyphenol pathways enabled resveratrol production directly from glucose. The construction of these C. glutamicum platform strains for the synthesis of plant polyphenols opens the door towards the microbial production of high-value aromatic compounds from cheap carbon sources with this microorganism.

Substances (12)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Resveratrol 501-36-0 C14H12O3 1360 suppliers $5.00-$5653.00
Caffeic acid 331-39-5 C9H8O4 925 suppliers $5.00-$1012.00
p-Coumaric acid 501-98-4 C9H8O3 638 suppliers $4.00-$2453.22
Naringenin 480-41-1 C15H12O5 565 suppliers $5.00-$6000.00
Cinnamic acid 621-82-9 C9H8O2 495 suppliers $6.00-$7596.38
PICEATANNOL 10083-24-6 C14H12O4 425 suppliers $5.00-$1610.00
D-Glucose monohydrate 5996-10-1 C6H14O7 393 suppliers $29.00-$6717.78
ERIODICTYOL 552-58-9 C15H12O6 292 suppliers $12.00-$970.00
PINOSYLVIN 22139-77-1 C14H12O2 236 suppliers $36.00-$1589.70
pekinense 14 suppliers Inquiry

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