一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Molecular medicine reports >>article
Molecular medicine reports

Molecular medicine reports

IF: 3.4
Download PDF

Proanthocyanidins exert a neuroprotective effect via ROS/JNK signaling in MPTP-induced Parkinson's disease models in vitro and in vivo

Published:1 December 2018 DOI: 10.3892/mmr.2018.9509 PMID: 30272275
Hucheng Chen, Jiyu Xu, Yuan Lv, Ping He, Chunyan Liu, Jie Jiao, Shiwei Li, Xuhua Mao, Xue Xue

Abstract

The pathological alterations of Parkinson's disease (PD) predominantly manifest as a loss of dopaminergic neurons in the substantia nigra, which may be caused by oxidative stress damage. Proanthocyanidins (PCs) are a class of compounds found in various plants, which have significant antioxidant and free radical?scavenging activity. The present study investigated the protective effects and underlying mechanisms of PCs in a 1?methyl?4?phenyl?1,2,3,6?tetrahydropyridine (MPTP)?induced PD model in?vitro and in?vivo. MTT assays were used to detect cell viability, and flow cytometry and TUNEL assays were used to detect cell apoptosis. Mitochondrial membrane potential (MMP) alterations were investigated using a JC?1 MMP Assay kit. The pole test was used to measure motor behavior in a mouse model of PD. Levels of reactive oxygen species (ROS) were measured using the fluorescent probe, 2',7'?dichlorodihydrofluorescein diacetate. Immunohistochemistry and western blotting were performed to detect the expression levels of proteins associated with PD. In?vitro, it was demonstrated that in MPTP?treated PC12 cells, PCs increased cell viability and reduced cell apoptosis in a dose?dependent manner. In?vivo, it was revealed that PC treatment inhibited striatal dopamine depletion, which resulted in significant improvements in PD?like movement impairment. Reactive oxygen species (ROS) production and MPTP?induced apoptosis were also inhibited. Furthermore, the results demonstrated that the neuroprotective activity of PCs may be mediated via the inhibition of ROS generation, as well as modulation of c?Jun N?terminal kinase activation. Taken together, these data revealed that PCs may exert neuroprotective effects in in?vivo and in?vitro PD models, and may have potential in the prevention or treatment of PD.

Similar articles

IF:5.4

Huperzine A and Its Neuroprotective Molecular Signaling in Alzheimer's Disease.

ACS Applied Energy Materials María Jesús Friedli, Nibaldo C Inestrosa,etc Published: 29 October 2021
IF:4.6

In Vitro and In Vivo Neuroprotective Effects of Sarcosine.

ACS Applied Bio Materials Arzugül Tanas, ?zlem ?zdemir Tozlu,etc Published: 15 October 2022
IF:98.4

Amantadine in Parkinson's disease.

The Lancet P Millac, I Hasan,etc Published: 28 February 1970
绵竹市| 翼城县| 广饶县| 剑阁县| 华宁县| 邳州市| 遵义县| 南华县| 永靖县| 屏东县| 马边| 清镇市| 新安县| 汶上县| 加查县| 儋州市| 左贡县| 宜兰市| 彩票| 佳木斯市| 新源县| 崇文区| 焉耆| 新民市| 龙岩市| 来凤县| 楚雄市| 正蓝旗| 扎赉特旗| 漯河市| 江都市| 杭锦旗| 梁河县| 修文县| 牙克石市| 铜梁县| 东阳市| 临邑县| 平湖市| 潞城市| 莲花县|