一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of biotechnology >>article
Journal of biotechnology

Journal of biotechnology

IF: 4.09
Download PDF

Efficient biosynthesis of (R)-3-amino-1-butanol by a novel (R)-selective transaminase from Actinobacteria sp.

Published:10 April 2019 DOI: 10.1016/j.jbiotec.2019.02.008 PMID: 30853639
Xiao-Ling Tang , Nan-Nan Zhang , Guo-Yan Ye , Yu-Guo Zheng

Abstract

(R)-3-amino-1-butanol is a key intermediate of Dolutegravir for the treatment of HIV/AIDS and its green and efficient biosynthesis has attracted a great deal of attention. Transaminases are currently used as promising biocatalyst for the synthesis of chiral amines. However, many transaminases have (S)-specificity and (R)-selective transaminases were less exploited and studied, making the production of (R)-amines remain challenging. In this study, a novel transaminase from Actinobacteria sp. (As-TA) was obtained and applied for the biosynthesis of (R)-3-amino-1-butanol by transferring the amino group from isopropylamine to 4-hydroxy-2-butanone. After optimization of the reaction condition and using a substrate fed-batch strategy, the conversion of 100, 200, 300, 400 and 500?mM 4-hydroxy-2-butanone reached 100%, 94.9%, 86.1%, 76.1% and 70.9%, respectively. (R)-3-amino-1-butanol with a maximum yield of 29.6?g/L and 99.9% e.e. value was obtained. This was the first time demonstrating the successful biosynthesis of (R)-3-amino-1-butanol with transaminase as biocatalyst and the obtained As-TA enriched the enzyme pool of transaminase with (R)-specificity.

Substances (4)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Isopropylamine 75-31-0 C3H9N 475 suppliers $7.62-$675.00
(R)-3-amino-1-butanol 61477-40-5 C4H11NO 461 suppliers $5.00-$1190.00
4-Hydroxy-2-butanone 590-90-9 C4H8O2 353 suppliers $7.62-$859.90
Granuliberin R peptide 7 suppliers Inquiry

Similar articles

IF:5.2

n-Butanol production by Rhodopseudomonas palustris TIE-1

Communications Biology Wei Bai, Tahina Onina Ranaivoarisoa,etc Published: 3 November 2021
IF:4.3

Acute effects of exposure to vapors of 3-methyl-1-butanol in humans.

Indoor air L Ernstg?rd, D Norb?ck,etc Published: 1 June 2013
郯城县| 金寨县| 青龙| 岳普湖县| 蒙阴县| 金平| 正镶白旗| 高阳县| 达拉特旗| 通道| 辽宁省| 水城县| 平顺县| 江西省| 工布江达县| 旬阳县| 容城县| 古交市| 清涧县| 柞水县| 盈江县| 平塘县| 娱乐| 巩义市| 孝感市| 崇义县| 星子县| 岳西县| 肇东市| 金平| 北流市| 金昌市| 东台市| 彭阳县| 山丹县| 惠水县| 金山区| 塘沽区| 太湖县| 孝昌县| 定西市|