一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Nature >>article
Nature

Nature

IF: 50.5
Download PDF

Time-resolved protein activation by proximal decaging in living systems.

Published:8 May 2019 DOI: 10.1038/s41586-019-1188-1 PMID: 31068699
Jie Wang, Yuan Liu, Yanjun Liu, Siqi Zheng, Xin Wang, Jingyi Zhao, Fan Yang, Gong Zhang, Chu Wang, Peng R. Chen

Abstract

A universal gain-of-function approach for selective and temporal control of protein activity in living systems is crucial to understanding dynamic cellular processes. Here we report development of a computationally aided and genetically encoded proximal decaging (hereafter, CAGE-prox) strategy that enables time-resolved activation of a broad range of proteins in living cells and mice. Temporal blockage of protein activity was computationally designed and realized by genetic incorporation of a photo-caged amino acid in proximity to the functional site of the protein, which can be rapidly removed upon decaging, resulting in protein re-activation. We demonstrate the wide applicability of our method on diverse protein families, which enabled orthogonal tuning of cell signalling and immune responses, temporal profiling of proteolytic substrates upon caspase activation as well as the development of protein-based pro-drug therapy. We envision that CAGE-prox will open opportunities for the gain-of-function study of proteins and dynamic biological processes with high precision and temporal resolution. The authors report a general strategy for in vivo protein activation using light-controlled proximal decaging directed by in silico screening.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
PENTOSTATIN 53910-25-1 C11H16N4O4 234 suppliers $38.00-$3282.70
PENTOSTATIN 53910-25-1 C11H16N4O4 234 suppliers $38.00-$3282.70
PENTOSTATIN 53910-25-1 C11H16N4O4 234 suppliers $38.00-$3282.70
PENTOSTATIN 53910-25-1 C11H16N4O4 234 suppliers $38.00-$3282.70

Similar articles

IF:8.3

Antibacterial Soy Protein Isolate Prepared by Quaternization.

ACS Applied Materials & Interfaces Qi Dong, Jingwen Lei,etc Published: 14 August 2022
通许县| 封开县| 财经| 泸定县| 莱阳市| 郓城县| 蚌埠市| 铁岭县| 北京市| 贡嘎县| 潼南县| 南川市| 灌南县| 八宿县| 商丘市| 阳原县| 景洪市| 施秉县| 利津县| 南岸区| 长丰县| 铜川市| 水富县| 阿拉善盟| 乐平市| 台中县| 香港 | 会东县| 大城县| 石渠县| 甘谷县| 阳谷县| 延安市| 山阳县| 五大连池市| 阿坝县| 静宁县| 文成县| 正蓝旗| 浑源县| 文化|