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Cell Research

Cell Research

IF: 25.9
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A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts

Published:3 July 2019 DOI: PMID: 31270412
Bingqing Xie, Da Sun, Yuanyuan Du, Jun Jia, Shicheng Sun, Jun Xu, Yifang Liu, Chengang Xiang, Sitong Chen, Huangfan Xie, Qiming Wang, Guangya Li, Xuehui LYU, Hui Shen, Shiyu Li, Min Wu, Xiaonan Zhang, Yue Pu, Kuanhui Xiang, Weifeng Lai, Peng Du, Zhenghong Yuan, Cheng Li, Yan Shi, Shichun Lu, Hongkui Deng

Abstract

Terminally differentiated cells can be generated by lineage reprogramming, which is, however, hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming.

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Materials
Procduct Name CAS Molecular Formula Supplier Price
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Lamivudine 134678-17-4 C8H11N3O3S 815 suppliers $5.00-$2400.00
Entecavir 142217-69-4 C12H15N5O3 555 suppliers $15.00-$4277.00
Entecavir 142217-69-4 C12H15N5O3 555 suppliers $15.00-$4277.00
Entecavir 142217-69-4 C12H15N5O3 555 suppliers $15.00-$4277.00
Entecavir 142217-69-4 C12H15N5O3 555 suppliers $15.00-$4277.00
Lamivudine 134678-17-4 C8H11N3O3S - Inquiry
Lamivudine 134678-17-4 C8H11N3O3S - Inquiry

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