一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Autophagy >>article
Autophagy

Autophagy

IF: 14.3
Download PDF

The protease activity of human ATG4B is regulated by reversible oxidative modification

Published:1 October 2020 DOI: 10.1080/15548627.2019.1709763 PMID: 31880198
Xueping Zheng, Zuolong Yang, Qianqian Gu, Fan Xia, Yuanyuan Fu, Peiqing Liu, Xiao-Ming Yin, Min Li

Abstract

Macroautophagy/autophagy plays a pivotal role in cytoplasmic material recycling and metabolic turnover, in which ATG4B functions as a "scissor" for processing pro-LC3 and lipidated LC3 to drive the autophagy progress. Mounting evidence has demonstrated the tight connection between ROS and autophagy during various pathological situations. Coincidentally, several studies have shown that ATG4B is potentially regulated by redox modification, but the underlying molecular mechanism and its relationship with autophagy is ambiguous. In this study, we verified that ATG4B activity was definitely regulated in a reversible redox manner. We also determined that Cys292 and Cys361 are essential sites of ATG4B to form reversible intramolecular disulfide bonds that respond to oxidative stress. Interestingly, we unraveled a new phenomenon that ATG4B concurrently formed disulfide-linked oligomers at Cys292 and Cys361, and that both sites underwent redox modifications thereby modulating ATG4B activity. Finally, increased autophagic flux and decreased oxidation sensitivity were observed in Cys292 and Cys361 double site-mutated cells under normal growth conditions. In conclusion, our research reveals a novel molecular mechanism that oxidative modification at Cys292 and Cys361 sites regulates ATG4B function, which modulates autophagy.Abbreviations: Air-ox: air-oxidation; ATG4B: autophagy related 4B cysteine peptidase; BCNU: 1,3-bis(2-chloroethyl)-1-nitrosourea; CBB: Coomassie Brilliant Blue; CM: complete medium; CQ: chloroquine; DTT: dithiothreitol; GSH: reduced glutathione; GSNO: S-nitrosoglutathione; GSSG: oxidized glutathione; HMW: high molecular weight; H2O2: hydrogen peroxide; NAC: N-acetyl-L-cysteine; NEM: N-ethylmaleimide; PE: phosphatidylethanolamine; PTM: post-translational modification; ROS, reactive oxygen species; WT: wild type.

Substances (7)

Materials Related products
Procduct Name CAS Molecular Formula Supplier Price
Auranofin 34031-32-8 C20H34AuO9PS 197 suppliers $45.00-$2596.00
Auranofin 34031-32-8 C20H34AuO9PS 197 suppliers $45.00-$2596.00
Auranofin 34031-32-8 C20H34AuO9PS 197 suppliers $45.00-$2596.00
Auranofin 34031-32-8 C20H34AuO9PS 197 suppliers $45.00-$2596.00
Auranofin 34031-32-8 - Inquiry
Auranofin 34031-32-8 - Inquiry

Similar articles

IF:5.7

Mineral oil in food, cosmetic products, and in products regulated by other legislations.

Critical Reviews in Toxicology Ralph Pirow, Annegret Blume,etc Published: 1 October 2019
IF:0.4

Dihydromyricetin Prevents Inflammation and Oxidative Stress in Human Osteoarthritis Chondrocytes

Current Topics in Nutraceutical Research P. Yao,?Jinwei Ai,etc Published: 1 September 2021
额敏县| 聊城市| 天峨县| 铜陵市| 平远县| 巴彦县| 临城县| 类乌齐县| 长岛县| 日土县| 孝感市| 宜宾县| 平远县| 梁山县| 阿拉善左旗| 梅河口市| 名山县| 屯留县| 平乐县| 措勤县| 新平| 绍兴市| 明光市| 金堂县| 冀州市| 河南省| 通榆县| 建湖县| 襄垣县| 工布江达县| 乐亭县| 棋牌| 隆子县| 云浮市| 泗水县| 商丘市| 邳州市| 称多县| 德化县| 新昌县| 天峨县|