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IF: 5.2
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The expression and role of glycolysis-associated molecules in infantile hemangioma

Published:15 October 2020 DOI: PMID: 32768579
Jian Chen , Dan Wu , Zuoqing Dong , Anwei Chen , Shaohua Liu

Abstract

Aims

Infantile hemangioma (IH) is one of the most common tumors in infancy, which etiology and pathogenesis has not been fully elucidated, hypoxia and abnormal glucose metabolism is regarded as critical pathogenic factors. This study investigated the expression and function of glycolysis-associated molecules (GLUT1, HK2, PFKFB3, PKM2, and LDHA) under normoxic and hypoxic conditions to further understand the pathogenesis of IH.

Main methods

Hemangioma-derived endothelial cells (HemECs) were isolated from proliferating phase infantile hemangiomas and identified by immunofluorescence. HemECs and human umbilical vein endothelial cells (HUVECs) were cultured under normoxic and hypoxic conditions. RNA and protein expression of glycolysis-associated molecules were analyzed by quantitative real-time RT-PCR, western blotting, and immunohistochemistry. Glucose consumption, ATP production and lactate production were measured. Glycolysis-associated molecules were inhibited by WZB117, 3BP, 3PO, SKN, and GSK 2837808A and the resulting effects on HemECs proliferation, migration, and tube formation were quantified.

Key findings

Glycolysis-associated molecules were highly expressed at both mRNA and protein levels in HemECs compared with HUVECs (P?<?0.05). Glucose consumption and ATP production were higher in HemECs than in HUVECs, while lactate production in HemECs was lower than in HUVECs (P?<?0.05). Inhibition of some glycolysis-associated molecules reduced the proliferation, migration, and tube formation capacity of HemECs (P?<?0.05).

Significance

Our study revealed that glycolysis-associated molecules were highly expressed in IH. Glucose metabolismin HemECs differed from normal endothelial cells. Altering the expression of glycolysis-associated molecules may influence the phenotype of HemECs and provide new therapeutic approaches to the successful treatment of IH.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
WZB117 1223397-11-2 C20H13FO6 114 suppliers $11.00-$1700.00
WZB117 1223397-11-2 C20H13FO6 114 suppliers $11.00-$1700.00
WZB117 1223397-11-2 C20H13FO6 114 suppliers $11.00-$1700.00
WZB117 1223397-11-2 C20H13FO6 114 suppliers $11.00-$1700.00

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