一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Life sciences >>article
Life sciences

Life sciences

IF: 5.2
Download PDF

Screened antipsychotic drugs inhibit SARS-CoV-2 binding with ACE2 in vitro

Published:1 February 2021 DOI: 10.1016/j.lfs.2020.118889 PMID: 33310043
Jiayu Lu, Yajing Hou, Shuai Ge, Xiangjun Wang, Jue Wang, Tian Hu, Yuexin Lv, Huaizhen He, Cheng Wang

Abstract

Aim

The coronavirus disease 2019 (COVID-19) pandemic has swept the globe and no specific effective drug has been identified. Drug repurposing is a well-known method to address the crisis in a time-critical fashion. Antipsychotic drugs (APDs) have been reported to inhibit DNA replication of hepatitis B virus, measles virus germination, and HIV infection, along with replication of SARS-CoV and MERS-CoV, both of which interact with host cells as SARS-CoV-2.

Methods

Nineteen APDs were screened using ACE2-HEK293T cell membrane chromatography (ACE2-HEK293T/CMC). Cytotoxicity assay, coronavirus spike pseudotype virus entry assay, surface plasmon resonance, and virtual molecular docking were applied to detect affinity between ACE2 protein and drugs and a potential antiviral property of the screened compounds.

Key findings

After the CMC screening, 8 of the 19 APDs were well-retained on ACE2-HEK293T/CMC column and showed significant antiviral activities in vitro. Three quarters of them belong to phenothiazine and could significantly inhibit the entrance of coronavirus into ACE2-HEK293T cells. Aother two drugs, aripiprazole and tiapride, exhibited weaker inhibition. We selected five of the drugs for subsequent evaluation. All five showed similar affinity to ACE2 and virtual molecular docking demonstrated they bound with different amino acids respectively on ACE2 which SARS-CoV-2 binds to.

Significance

Eight APDs were screened for binding with ACE2, five of which demonstrated potential protective effects against SARS-CoV-2 through acting on ACE2. Although the five drugs have a weak ability to block SARS-CoV-2 with a single binding site, they may provide a synergistic effect in adjuvant therapy of COVID-19 infection.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Trifluoperazine 117-89-5 C21H24F3N3S 84 suppliers Inquiry
Trifluoperazine 117-89-5 C21H24F3N3S 84 suppliers Inquiry
Trifluoperazine 117-89-5 C21H24F3N3S 84 suppliers Inquiry
Trifluoperazine 117-89-5 C21H24F3N3S 84 suppliers Inquiry
深圳市| 开化县| 正镶白旗| 永嘉县| 临洮县| 隆尧县| 黔西县| 平昌县| 阿瓦提县| 襄垣县| 梓潼县| 大邑县| 南丰县| 浮梁县| 西峡县| 乌拉特前旗| 永嘉县| 灌云县| 大邑县| 西林县| 驻马店市| 根河市| 香港 | 萍乡市| 丹棱县| 定南县| 拉萨市| 额济纳旗| 天津市| 南漳县| 化州市| 连城县| 蓬莱市| 长春市| 黎川县| 专栏| 福安市| 诸城市| 合水县| 乐清市| 南充市|