一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Analyst >>article
Analyst

Analyst

IF: 3.6
Download PDF

Enzyme-activated anchoring of peptide probes onto plasma membranes for selectively lighting up target cells

Published:13 April 2020 DOI: 10.1039/D0AN00487A PMID: 32350495
Julan Xia, Sujuan Sun, Xianhua Wu, Yan Huang, Chunyang Lei and Zhou Nie

Abstract

In a cellular microenvironment, numerous biomolecules are involved in various physiological and pathological processes. However, for the in-depth and comprehensive understanding of their roles at the molecular level, there is still a lack of detection techniques for the in situ tracking of these biomolecules in a local environment. Herein, we engineered a membrane insertion peptide (MIP) as an enzyme-activated membrane insertion peptide probe (eaMIP) that allowed the in situ tracking of the activity of target enzymes in living cells. In this strategy, the membrane insertion capacity of the MIP motif in each eaMIP was caged by appending a chemical moiety. In the presence of target enzymes, the caging moiety in each eaMIP was removed by enzymatic decaging, leading to the generation of active MIPs. The versatility of this design was demonstrated by lighting up different tumor cells with distinct fluorescence signal patterns, affording an alternative tool for clinical diagnostics, biochemical research and membrane engineering.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Ilomastat 142880-36-2 C20H28N4O4 171 suppliers $19.00-$5661.00
Ilomastat 142880-36-2 C20H28N4O4 171 suppliers $19.00-$5661.00
Ilomastat 142880-36-2 C20H28N4O4 171 suppliers $19.00-$5661.00
Ilomastat 142880-36-2 C20H28N4O4 171 suppliers $19.00-$5661.00

Similar articles

IF:5.4

Embedded optofluidic biosensing platform for enzyme-linked immunosorbent assay

Sensing and Bio-Sensing Research Jie Zhang, Yuyao Li,etc Published: 19 November 2024
景东| 竹溪县| 旺苍县| 镇沅| 邻水| 泰和县| 莲花县| 漳浦县| 土默特左旗| 普兰店市| 奈曼旗| 论坛| 浪卡子县| 越西县| 广西| 中宁县| 隆尧县| 贞丰县| 庆城县| 万山特区| 民县| 黑龙江省| 白河县| 大同县| 忻城县| 固安县| 新巴尔虎左旗| 仁布县| 富源县| 常州市| 额敏县| 泸州市| 上思县| 万山特区| 延长县| 大方县| 普安县| 北安市| 天长市| 安岳县| 固始县|