一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Life sciences >>article
Life sciences

Life sciences

IF: 5.2
Download PDF

TNF-α up-regulates Nanog by activating NF-κB pathway to induce primary rat spinal cord astrocytes dedifferentiation

Published:15 December 2021 DOI: 10.1016/j.lfs.2021.120126 PMID: 34758295
Zhenfei Ding , Ce Dai , Wenshan Shan , Rui Liu , Wei Lu , Weilu Gao , Hui Zhang , Wei Huang , Jianzhong Guan , Zongsheng Yin

Abstract

Aims

Astrocytes re-acquire stem cell potential upon inflammation, thereby becoming a promising source of cells for regenerative medicine. Nanog is an essential transcription factor to maintain the characteristics of stem cells. We aimed to investigate the role of Nanog in astrocyte dedifferentiation.

Main methods

TNF-α was used to induce the dedifferentiation of primary rat spinal cord astrocytes. The expression of immature markers CD44 and Musashi-1 was detected by qRT-PCR and immunofluorescence. The Nanog gene is knocked down by small interference RNA. Nanog expression was measured by qRT-PCR and western blotting. BAY 11–7082 was used to suppress NF-κB signals in astrocytes. NF-κB signaling was evaluated by Western blotting.

Key findings

Our results showed that TNF-α promoted the re-expression of CD44 and Musashi-1 in astrocytes. Dedifferentiated astrocytes could be induced to differentiate into oligodendrocyte lineage cells indicating that the astrocytes had pluripotency. In addition, TNF-α treatment activated NF-κB signaling pathway and up-regulated Nanog. Knockdown of Nanog reversed the increase of CD44 and Musashi-1 induced by TNF-α without affecting the activation of NF-κB signaling. Importantly, blocking NF-κB signaling by BAY 11–7082 inhibited the expression of immature markers suggesting that TNF-α induces dedifferentiation of astrocytes through the NF-κB signaling pathway. BAY 11–7082 could also inhibit the expression of Nanog, which indicated that Nanog was regulated by NF-κB signaling pathway.

Significance

These findings indicate that activation of the NF-κB signaling pathway through TNF-α leads to astrocytes dedifferentiation via Nanog. These results expand our understanding of the mechanism of astrocytes dedifferentiation.

Similar articles

IF:4.2

Tianeptine sodium salt suppresses TNF-α-induced expression of matrix metalloproteinase-9 in human carcinoma cells via suppression of the PI3K/Akt-mediated NF-κB pathway

Environmental toxicology and pharmacology Rajapaksha Gedara Prasad Tharanga Jayasooriya , Matharage Gayani Dilshara ,etc Published: 1 September 2014
IF:5.6

D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway

Food Science and Human Wellness Chong Wang, Fangting Wang,etc Published: 1 September 2022
IF:2.6

Sphingosine-1-phosphate receptor 3 promotes neuronal apoptosis via the TNF-α/caspase-3 signaling pathway after acute intracerebral hemorrhage

Molecular and Cellular Neuroscience Dengpan Song, Mengyuan Li,etc Published: 22 November 2024
平安县| 溧阳市| 札达县| 郧西县| 江源县| 牙克石市| 蕲春县| 资源县| 宜兰市| 喀什市| 开封县| 渝北区| 连城县| 新化县| 开远市| 育儿| 工布江达县| 孙吴县| 潞西市| 普陀区| 宁远县| 从化市| 唐河县| 高清| 华坪县| 泽库县| 吉隆县| 册亨县| 雅江县| 辰溪县| 房山区| 陕西省| 临朐县| 固安县| 施甸县| 潮安县| 财经| 澄江县| 呼玛县| 小金县| 孟村|