一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Acta Biomaterialia >>article
Acta Biomaterialia

Acta Biomaterialia

IF: 9.6
Download PDF

Substrate nesting guides cyst morphogenesis of human pluripotent stem cells without 3D extracellular matrix overlay

Published:1 September 2023 DOI: 10.1016/j.actbio.2023.08.053
Yun-Ping Chen , Yue Shao , Peng-Cheng Chen , Kun Li, Jing-Yi Li, Jie Meng, Cheng-Lin Lv, Hao-Yu Liu, Cunjing Lv, Xi-Qiao Feng, Bo Li

Abstract

Understanding the principles underlying the self-organization of stem cells into tissues is fundamental for deciphering human embryo development. Here, we report that, without three-dimensional (3D) extracellular matrix (ECM) overlay, human pluripotent stem cells (hPSCs) cultured on two-dimensional soft elastic substrates can self-organize into 3D cysts resembling the human epiblast sac in a stiffness-dependent manner. Our theoretical modeling predicts that this cyst organization is facilitated and guided by the spontaneous nesting of the soft substrate, which results from the adhesion-dependent mechanical interaction between cells and substrate. Such substrate nesting is sufficient for the 3D assembly and polarization of hPSCs required for cyst organization, even without 3D ECM overlay. Furthermore, we identify that the reversible substrate nesting and cyst morphogenesis also require appropriate activation of ROCK-Myosin II pathway. This indicates a unique set of tissue morphomechanical signaling mechanisms that clearly differ from the canonical cystogenic mechanism previously reported in 3D ECM. Our findings highlight an unanticipated synergy between mechanical microenvironment and mechanotransduction in controlling tissue morphogenesis and suggest a mechanics-based strategy for generation of hPSCs-derived models for early human embryogenesis.

Statement of significance

Soft substrates can induce the self-organization of human pluripotent stem cells (hPSCs) into cysts without three-dimensional (3D) extracellular matrix (ECM) overlay. However, the underlying mechanisms by which soft substrate guides cystogenesis are largely unknown. This study shows that substrate nesting, resulting from cell–substrate interaction, plays an important role in cyst organization, including 3D assembly and apical–basal polarization. Additionally, actomyosin contractility mediated by the ROCK-Myosin II pathway also contributes to the substrate deformation and cyst morphology. These findings demonstrate the interplay between the mechanical microenvironment and cells in tissue morphogenesis, suggesting a mechanics-based strategy in building hPSC-derived models for early human embryo development.

Substances (4)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Y-27632 DIHYDROCHLORIDE 146986-50-7 C14H21N3O 277 suppliers $16.00-$2691.10
Y-27632 DIHYDROCHLORIDE 146986-50-7 C14H21N3O 277 suppliers $16.00-$2691.10
Y-27632 DIHYDROCHLORIDE 146986-50-7 C14H21N3O 277 suppliers $16.00-$2691.10
Y-27632 DIHYDROCHLORIDE 146986-50-7 C14H21N3O 277 suppliers $16.00-$2691.10

Similar articles

IF:4.2

Tianeptine sodium salt suppresses TNF-α-induced expression of matrix metalloproteinase-9 in human carcinoma cells via suppression of the PI3K/Akt-mediated NF-κB pathway

Environmental toxicology and pharmacology Rajapaksha Gedara Prasad Tharanga Jayasooriya , Matharage Gayani Dilshara ,etc Published: 1 September 2014
平舆县| 乌鲁木齐市| 楚雄市| 宣武区| 子洲县| 海原县| 临漳县| 子洲县| 方城县| 彰武县| 景宁| 搜索| 文昌市| 响水县| 新营市| 砚山县| 沁源县| 永昌县| 芜湖县| 榆树市| 黑水县| 体育| 太和县| 天气| 晋中市| 娄烦县| 溧水县| 大连市| 曲麻莱县| 平远县| 襄城县| 淮滨县| 双流县| 兴宁市| 金山区| 大宁县| 威宁| 延川县| 乐平市| 碌曲县| 云安县|