一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> American journal of physiology. Endocrinology and metabolism >>article

Spatiotemporally quantitative in vivo imaging of mitochondrial fatty acid β-oxidation at cellular-level resolution in mice

Published:1 November 2023 DOI: 10.1152/ajpendo.00147.2023 PMID: 37729022
Ayumi Matsumoto, Isao Matsui, Shohei Uchinomiya, Yusuke Katsuma, Seiichi Yasuda, Hiroki Okushima, Atsuhiro Imai, Takeshi Yamamoto, Akio Ojida, Kazunori Inoue, Yoshitaka Isaka

Abstract

Mitochondrial fatty acid β-oxidation (FAO) plays a key role in energy homeostasis. Several FAO evaluation methods are currently available, but they are not necessarily suitable for capturing the dynamics of FAO in vivo at a cellular-level spatial resolution and seconds-level time resolution. FAOBlue is a coumarin-based probe that undergoes β-oxidation to produce a fluorescent substrate, 7-hydroxycoumarin-3-(N-(2-hydroxyethyl))-carboxamide (7-HC). After confirming that 7-HC could be specifically detected using multiphoton microscopy at excitation/emission wavelength = 820/415-485 nm, wild-type C57BL/6 mice were randomly divided into control, pemafibrate, fasting (24 or 72 h), and etomoxir groups. These mice received a single intravenous injection of FAOBlue. FAO activities in the liver of these mice were visualized using multiphoton microscopy at 4.2 s/frame. These approaches could visualize the difference in FAO activities between periportal and pericentral hepatocytes in the control, pemafibrate, and fasting groups. FAO velocity, which was expressed by the maximum slope of the fluorescence intensity curve, was accelerated in the pemafibrate and 72-h fasting groups both in the periportal and the pericentral hepatocytes in comparison with the control group. Our approach revealed differences in the FAO activation mode by the two stimuli, i.e., pemafibrate and fasting, with pemafibrate accelerating the time of first detection of FAO-derived fluorescence. No increase in the fluorescence was observed in etomoxir-pretreated mice, confirming that FAOBlue specifically detected FAO in vivo. Thus, FAOBlue is useful for visualizing in vivo liver FAO dynamics at the single-cell-level spatial resolution and seconds-level time resolution.NEW & NOTEWORTHY Fatty acid β-oxidation (FAO) plays a key role in energy homeostasis. Here, the authors established a strategy for visualizing FAO activity in vivo at the cellular-level spatial resolution and seconds-level time resolution in mice. Quantitative analysis revealed spatiotemporal heterogeneity in hepatic FAO dynamics. Our method is widely applicable because it is simple and uses a multiphoton microscope to observe the FAOBlue-injected mice.

Substances (1)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Pemafibrate 848259-27-8 C28H30N2O6 146 suppliers Inquiry

Similar articles

IF:5

Fatigue-resistant hydrogels controlled by sulfanilic acid cross-linker at molecular level

Polymer Testing Zhenming Qi, Xiaosai Hu,etc Published: 1 February 2022
IF:0

Gestational diabetes mellitus is associated with a low serum level of mitochondrial-derived peptide-MOTS-C

Turk biyokimya dergisi = Turkish journal of biochemistry Serdar ?zer, Filiz Alkan Baylan,etc Published: 21 September 2023
IF:0

Chemical Kinetic Study on Reaction Pathway of Anisole Oxidation at Various Operating Condition

Volume 8: Energy Shrabanti Roy, Omid Askari,etc Published: 16 November 2020
东平县| 富裕县| 昆明市| 深州市| 原平市| 隆林| 哈密市| 八宿县| 江安县| 金山区| 孟村| 兴业县| 凉城县| 台前县| 丽江市| 福海县| 永清县| 九台市| 扶余县| 进贤县| 来宾市| 山丹县| 汾阳市| 凤城市| 兴隆县| 乌拉特后旗| 宁明县| 安阳县| 克山县| 体育| 马尔康县| 灵山县| 耿马| 合川市| 岳池县| 蓬莱市| 武平县| 桃园县| 资兴市| 正定县| 韶关市|