一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Journal of Proteome Research >>article
Journal of Proteome Research

Journal of Proteome Research

IF: 3.6
Download PDF

Identification of a USP9X Substrate NFX1-123 by SILAC-Based Quantitative Proteomics

Published:6 May 2019 DOI: PMID: 31059266
Xiangling Chen, Dayun Lu, Jing Gao, Hongwen Zhu, Yanting Zhou, Daming Gao, Hu Zhou*

Abstract

The deubiquitinase USP9X is involved in multiple diseases including neurodegeneration, epilepsy, and various types of tumors by targeting different substrates. In the present study, we aimed to explore the potential substrates of USP9X and performed SILAC-based quantitative proteomics to compare these substrates in USP9X-knockdown and wild-type HeLa cells. We consequently carried out Flag-NFX1-123 tag affinity-based mass spectrometry and confirmed that the X-box binding nuclear factor NFX1-123 interacted with USP9X. Moreover, immunoprecipitation assays verified a direct interaction between USP9X and NFX1-123. Further experiments confirmed that NFX1-123 could be modified by ubiquitination and that USP9X stabilized NFX1-123 via efficient deubiquitination of NFX1-123. Knockdown of USP9X resulted in decreased NFX1-123 protein levels compared with their unchanged corresponding mRNA levels in different cell lines. In summary, we found that NFX1-123 was a bona fide substrate of the deubiquitinase USP9X and that it could be degraded by the ubiquitin-proteasome system. The present study provided new insight into understanding the biological function of USP9X by targeting its substrate NFX1-123.

Similar articles

IF:3.1

A Quantitative Assay of Sodium Triacetoxyborohydride

Organic Process Research & Development Michael J. Zacuto*, Joseph Perona,etc Published: 7 August 2019
IF:16.1

Dimethylamine as a Substrate in Hydroaminoalkylation Reactions

Angewandte Chemie International Edition M.?Sc. Jens Bielefeld, Prof.?Dr. Sven Doye,etc Published: 10 October 2017
IF:3.1

Quantitative GC determination of sodium triacetoxyborohydride (STAB)

Journal of pharmaceutical and biomedical analysis Ian Hale , James Chadwick ,etc Published: 5 September 2021
乌鲁木齐市| 扎囊县| 竹山县| 宁都县| 潮安县| 县级市| 富顺县| 原阳县| 阿拉善左旗| 尼勒克县| 湄潭县| 京山县| 双峰县| 金山区| 清涧县| 苏尼特右旗| 博爱县| 绵阳市| 且末县| 留坝县| 余江县| 亚东县| 仁怀市| 察雅县| 宝应县| 宁化县| 乌鲁木齐市| 正镶白旗| 凉山| 开远市| 佛学| 收藏| 元朗区| 健康| 海城市| 绍兴市| 咸阳市| 张家口市| 清水河县| 文化| 绥芬河市|