一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

ChemicalBook >> journal list >> Metabolic brain disease >>article
Metabolic brain disease

Metabolic brain disease

IF: 3.2
Download PDF

Metformin attenuates sevoflurane-induced neurogenesis damage and cognitive impairment: involvement of the Nrf2/G6PD pathway

Published:1 August 2023 DOI: 10.1007/s11011-023-01218-2 PMID: 37119382
Pei Fan,?Yuying Lu,?Haidong Wei,?Kui Wang,?Pengyu Jia,?Yuanyuan Zhang,?Yan Zhang,?Tianyue Wang,?Liufei Yang,?Jing Zhao,?Shuyue Zhang,?Haixia Lu,?Xinlin Chen,?Yong Liu,?Pengbo Zhang

Abstract

Anesthetics such as sevoflurane are commonly administered to infants and children. However, the possible neurotoxicity caused by prolonged or repetitive exposure to it should be a concern. The neuroprotective effects of metformin are observed in many models of neurological disorders. In this study, we investigated whether metformin could reduce the developmental neurotoxicity induced by sevoflurane exposure in neonatal rats and the potential mechanism. Postnatal day 7 (PND 7) Sprague-Dawley rats and neural stem cells (NSCs) were treated with normal saline or metformin before sevoflurane exposure. The Morris water maze (MWM) was used to observe spatial memory and learning at PND 35-42. Immunofluorescence staining was used to detect neurogenesis in the subventricular zone (SVZ) of the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus at PND 14. MTT assays, immunofluorescence staining, and TUNEL staining were used to assess the viability, proliferation, differentiation, and apoptosis of NSCs. Western blotting and ELISA were used to assess the protein expression of cleaved caspase-3, nuclear factor erythroid 2-related factor 2 (Nrf2), and glucose-6-phosphate dehydrogenase (G6PD) pathway-related molecules. Exposure to sevoflurane resulted in late cognitive defects, impaired neurogenesis in both the SVZ and SGZ, reduced NSC viability and proliferation, increased NSC apoptosis, and decreased protein expression of G6PD in vitro. Metformin pretreatment attenuated sevoflurane-induced cognitive functional decline and neurogenesis inhibition. Metformin pretreatment also increased the protein expression of Nrf2 and G6PD. However, treatment with the Nrf2 inhibitor, ML385 or the G6PD inhibitor, dehydroepiandrosterone (DHEA) reversed the protective effect of metformin on sevoflurane-induced NSC damage in vitro. Our findings suggested that metformin could reduce sevoflurane-induced neurogenesis damage and neurocognitive defects in the developing rat brain by influencing the Nrf2/G6PD signaling pathways.

Substances (18)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Glutathione 70-18-8 C10H17N3O6S 1444 suppliers $6.00-$3580.00
Glutathione 70-18-8 C10H17N3O6S 1444 suppliers $6.00-$3580.00
Glutathione 70-18-8 C10H17N3O6S 1444 suppliers $6.00-$3580.00
Glutathione 70-18-8 C10H17N3O6S 1444 suppliers $6.00-$3580.00
Metformin 657-24-9 C4H11N5 224 suppliers $5.00-$305754.00
Metformin 657-24-9 C4H11N5 224 suppliers $5.00-$305754.00
Metformin 657-24-9 C4H11N5 224 suppliers $5.00-$305754.00
Metformin 657-24-9 C4H11N5 224 suppliers $5.00-$305754.00
ML385 846557-71-9 C29H25N3O4S 158 suppliers $18.00-$2646.00
ML385 846557-71-9 C29H25N3O4S 158 suppliers $18.00-$2646.00

Similar articles

库车县| 望城县| 枣阳市| 东乡族自治县| 金山区| 永靖县| 万安县| 红原县| 嘉黎县| 江阴市| 灵武市| 曲阳县| 准格尔旗| 兰考县| 南川市| 东莞市| 长宁县| 怀化市| 茂名市| 周宁县| 翁牛特旗| 宣汉县| 彰化市| 蓝田县| 楚雄市| 平顶山市| 开平市| 蓬溪县| 泰来县| 顺义区| 赫章县| 绥宁县| 体育| 内黄县| 贵阳市| 高邮市| 理塘县| 临西县| 柳河县| 秦皇岛市| 奇台县|