1-PHENYL-1-CYCLOPROPANOL synthesis
- Product Name:1-PHENYL-1-CYCLOPROPANOL
- CAS Number:29526-96-3
- Molecular formula:C9H10O
- Molecular Weight:134.18
1314495-37-8
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Yield:-
Reaction Conditions:
with phosphite;Pseudomonas fluorescens 4-hydroxyacetophenone monooxygenase;phosphite dehydrogenase;oxygen;NADPH in dimethyl sulfoxide; pH=7.5 at 25; for 24 h;aq. buffer;Enzymatic reaction;
Steps:
4.7. General procedure for enzymatic oxidation reactions
General procedure: To a flask (2 mL, Eppendorf containing a solution of the starting material (1 M in DMSO, 5 μL) were added Tris/HCl buffer at pH 7.5 (50 mM, 440 μL), phosphite solution (500 mM, 20 μL), NADPH (100 mM, 10 μL), PTDH (100 μM, 5 μL), and enzyme (BVMO) (100 μM, 20 μL). Reactions were shaken at 200 rpm and 30 °C (PAMO and M446G PAMO mutant) or 150 rpm and 25 °C (HAPMO and CHMO) for the time established. The reactions were stopped, extracted with EtOAc (3 × 0.5 mL), dried over Na2SO4, and analyzed by chiral HPLC. Control experiments in the absence of an enzyme were performed for all substrates tested, and no reaction was observed.
References:
Brondani, Patricia B.;Dudek, Hanna;Reis, Joel S.;Fraaije, Marco W.;Andrade, Leandro H. [Tetrahedron Asymmetry,2012,vol. 23,# 9,p. 703 - 708] Location in patent:experimental part
93-58-3
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$13.50/100 mL
925-90-6
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$12.00/5ml
29526-96-3
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93-58-3
515 suppliers
$13.50/100 mL
2386-64-3
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$45.00/5ml
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