2'-Deoxyuridine synthesis
- Product Name:2'-Deoxyuridine
- CAS Number:951-78-0
- Molecular formula:C9H12N2O5
- Molecular Weight:228.2
951-77-9
447 suppliers
$5.00/100mg
951-78-0
517 suppliers
$5.00/100mg
Yield:951-78-0 99%
Reaction Conditions:
with cytidine deaminase enzyme in aq. phosphate buffer at 37; pH=7; for 0.0833333 h;Enzymatic reaction;
Steps:
3 Comparative Example 3: Deamination of 2'-deoxycytidine to 2'-deoxyuridine
Comparative Example 3: Deamination of 2'-deoxycytidine to 2'-deoxyuridine A 100 mM solution of 2'-deoxycytidine (495 μΙ_) in 100 mM phosphate buffer at pH 7 was mixed with 50 μΙ_ of cytidine deaminase enzyme solution containing >300 AU in phosphate buffer. The reaction was performed at 37°C during 5 minutes and stopped with HCI. Then, the crude reaction was filtered through a 10 KDa membrane, and a portion was diluted and analyzed by HPLC-UV-DAD. Product identification was performed by comparison to a standard sample. 2'-Deoxyuridine was obtained in quantitative yield (>99%).
References:
WO2016/146808,2016,A1 Location in patent:Page/Page column 29
98495-56-8
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$70.00/500mg
951-78-0
517 suppliers
$5.00/100mg
4753-04-2
71 suppliers
$95.00/100mg
951-78-0
517 suppliers
$5.00/100mg
64911-18-8
20 suppliers
$124.00/25mg
951-78-0
517 suppliers
$5.00/100mg
