PENTYL VALERATE synthesis
- Product Name:PENTYL VALERATE
- CAS Number:2173-56-0
- Molecular formula:C10H20O2
- Molecular Weight:172.26
71-41-0
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$9.33/100 mL
109-52-4
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$10.60/1gm:
2173-56-0
118 suppliers
$25.00/25mL
Yield:2173-56-0 88%
Reaction Conditions:
with Pseudomonas sp. DMVR46 lipase immobilized on sodium bis(2-ethylhexyl)sulfosuccinate-based organogel in cyclohexane; pH=8.5 at 37; for 96 h;Enzymatic reaction;Reagent/catalyst;
Steps:
2.8 Synthesis of pentyl valerate under water restricted environment using free and immobilized DMVR46 lipase
Pentyl valerate was synthesized by the condensation of pentanol and valeric acid using free as well as immobilized lipase as described by Dandavate et al. [23]. For immobilization, the partially purified lipase was first entrapped in a surfactant solution comprising of 0.1M sodium bis (2-ethylhexyl) sulfosuccinate (AOT) with Wo=60 in isooctane by vigorously mixing the two solutions to create a clear reverse micellar system of AOT/50mM phosphate buffer (pH 8.5) - lipase/isooctane (1mL). The amount of purified lipase used for immobilization was 108μL having specific activity of 64.57U/mg. This system was then polymerized by the addition of 14% gelatin (1.5mL) maintained at 55°C followed by vigorous mixing to obtain homogenous AOT based organogel (MBGs). The gel was poured into plastic Petri plates, dried overnight, and cut into small pieces (1cm×1cm with 110±10μm thickness). The reaction mixture consisted of 20mL cyclohexane and equimolar concentrations (100mM) of pentanol and valeric acid. The esterification reaction was initiated by addition of free lipase and the pieces of MBGs to the reaction mixture in glass-stoppered flasks kept on orbital shaker at 37°C and 150rpm. At every 24h interval 100μL of the reaction mixture was withdrawn and analyzed by gas chromatograph.
References:
Patel, Vrutika;Nambiar, Shruti;Madamwar, Datta [Process Biochemistry,2014,vol. 49,# 10,p. 1673 - 1681]
71-41-0
456 suppliers
$9.33/100 mL
2173-56-0
118 suppliers
$25.00/25mL
71-41-0
456 suppliers
$9.33/100 mL
123-76-2
495 suppliers
$5.00/25g
2173-56-0
118 suppliers
$25.00/25mL
71-41-0
456 suppliers
$9.33/100 mL
110-62-3
263 suppliers
$13.44/25ML
2173-56-0
118 suppliers
$25.00/25mL
108-29-2
376 suppliers
$10.00/5 g
2173-56-0
118 suppliers
$25.00/25mL
109-52-4
359 suppliers
$10.60/1gm: