一二三四区视频,亚洲少妇熟女色,日本久热无码视频网,欧美国产日韩大尺度,亚洲a视频,久久少妇一区二区,日韩999无码视频,刺激久久久久久久,啊啊啊啊不要啊在线

Welcome to chemicalbook!
Chinese English Japanese Germany Korea
010-86108875
Try our best to find the right business for you.
Do not miss inquiry messages Please log in to view all inquiry messages.

Welcome back!

ChemicalBook CAS DataBase List Sagittatosdie B
118525-36-3

Sagittatosdie B synthesis

1synthesis methods
-

Yield:118525-36-3 96.4%

Reaction Conditions:

with Cunninghamella blakesleana in methanol; for 120 h;Microbiological reaction;regioselective reaction;

Steps:

2.3 Culture and biotransformation procedures

General procedure: Mycelia of C. blakesleeana from agar slants (2cm2) were transferred to 100mL of medium and cultured at 30°C with 175rpm for 36h to make a stock inoculum. Then 10mL volume of the inoculum was added to 1000mL of liquid medium, and the flask was placed on rotary shaker operating at 175rpm and 30°C. After 36h of preculture, 100mg of icariin with 2mL methanol added into 2L of culture medium for preparative biotransformation with the final concentration of 50mg/L. The incubation was continued for 5 additional days. The culture was filtered, and the filtrate was extracted with same volume of EtOAc for five times. And the suspension cells of C. blakesleeana (5.2g) were also extracted by using EtOAc (1000mL). Then all extraction of EtOAc were collected and concentrated under the reduced pressure. The residues (1.6g) were applied to an ODS column (200g, 10cm×50cm) and eluted with MeOH-H2O (in a gradient manner from 10% to 90%, at a flow rate of 2.0mL/min). Totally, 90.1mg of icariside II (96.5% purity) was obtained from fraction of 60% MeOH (500mL).

References:

Xin, XiuLan;Fan, Guang-Jun;Sun, Zheng;Zhang, Ning;Li, Ye;Lan, Rong;Chen, Liang;Dong, PeiPei [Journal of Molecular Catalysis B: Enzymatic,2015,vol. 122,p. 141 - 146]

Sagittatosdie B Related Search:

七台河市| 堆龙德庆县| 皋兰县| 利川市| 博野县| 桑日县| 河南省| 新丰县| 平安县| 仲巴县| 宁夏| 东乡族自治县| 固安县| 察雅县| 财经| 三亚市| 西乌珠穆沁旗| 安庆市| 广南县| 吉安市| 宜良县| 新宁县| 阿城市| 长泰县| 建始县| 黑水县| 南城县| 灵丘县| 忻城县| 阜城县| 武邑县| 大田县| 沁水县| 五华县| 昌都县| 盖州市| 镶黄旗| 苏尼特右旗| 河西区| 天台县| 新密市|