151519-02-7
151519-02-7 結(jié)構(gòu)式
基本信息
2H-Pyran-2-one, 5-ethyl-5,6-dihydro-6-[(2R,3S,4R,5S,7E)-2-hydroxy-4-methoxy-3,5-dimethyl-7-nonen-1-yl]-, (5R,6R)-
物理化學性質(zhì)
應用領域
以5-甲基-2-庚烯為原料經(jīng)以下步驟合成：
水稻以100g/10公畝劑量處理水稻，對其地面部分抑制程度為18%～23%，以25g/10公畝劑量處理，則對地面部分幾乎無抑制作用，在出穗前5～9d施用pironetin對產(chǎn)量無影響。小麥用125~1000mg/L的噴灑濃度處理旱田小麥，對株高呈現(xiàn)20%左右的生長抑制活性，但對小麥穗數(shù)并無影響。以2000mg/L處理對小麥有藥害，平均每穗重及千粒重減少10%左右。
常見問題列表
雄小鼠急性經(jīng)口 LD500325mg/kg。Ames試驗呈陰性。
Microtubule
Pironetin (20-100 ng/mL; 24 hours; 3Y1 cells) treatment arrests the cell cycle progression at G2/M in 3Y1 cells.
Pironetin (1-10000 ng/mL; 3 days; HeLa, A2780 and K-NRK cells) treatment inhibits the cell proliferation.
IC
50
values against these cell lines are almost 10 ng/mL.
Cell Cycle Analysis
| Cell Line: | 3Y1 cells |
| Concentration: | 20 ng/mL, 50 ng/mL, 100 ng/mL |
| Incubation Time: | 24 hours |
| Result: | Arrested the cell cycle progression at G2/M in3Y1 cells. |
Cell Proliferation Assay
| Cell Line: | HeLa, A2780 and K-NRK cells |
| Concentration: | 1 ng/mL, 10 ng/mL, 100 ng/mL, 1000 ng/mL and 10000 ng/mL |
| Incubation Time: | 3 days |
| Result: | Inhibited the cell proliferation. |
Pironetin (0.78-6.25 mg/kg; intraperitoneal injection; daily; for 5 days; female CDF1-SLC mice) treatment shows a moderate antitumor effect, however, a severe weight loss is observed as a side effect.
| Animal Model: | Female CDF1-SLC mice (10 weeks) injected with P388 murine leukemia cells |
| Dosage: | 0.78 mg/kg, 1.56 mg/kg, 3.13 mg/kg, 6.25 mg/kg |
| Administration: | Intraperitoneal injection; daily; for 5 days |
| Result: | Showed a moderate antitumor effect. |